TasA

• Description: major component of biofilm matrix, forms amyloid fibers
  
  

Gene name	tasA
Synonyms	cotN, yqhF
Essential	no
Product	major component of biofilm matrix
Function	biofilm formation
Gene expression levels in SubtiExpress: tasA
Interactions involving this protein in SubtInteract: TasA
Regulation of this protein in SubtiPathways: tasA
MW, pI	28 kDa, 5.442
Gene length, protein length	783 bp, 261 aa
Immediate neighbours	sinR, sipW
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biofilm formation

This gene is a member of the following regulons

AbrB regulon, RemA regulon, SinR regulon

The gene

Basic information

• Locus tag: BSU24620

Phenotypes of a mutant

• altered cell death pattern in colonies PubMed

Database entries

• BsubCyc: BSU24620

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: forms amyloid fibers that bind cells together in the biofilm PubMed

• Protein family: peptidase M73 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • forms fibers of variable length and 10-15 nm in width PubMed
  • TapA-TasA PubMed
  • SipW-TasA PubMed

• Localization:
  • extracellular (signal peptide) PubMed, secretion requires SipW PubMed
  • membrane PubMed

Database entries

• BsubCyc: BSU24620

• Structure:

• UniProt: P54507

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tapA-sipW-tasA PubMed

• Expression browser: tasA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • repressed by SinR PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed
  • SinR: transcription repression PubMed
  • RemA: transcription activation PubMed

• Additional information:
  • induction by sequestration of SinR by SinI or SlrA PubMed or by SlrR PubMed
  • the tapA-sipW-tasA operon is not expressed in a ymdB mutant PubMed
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y (this is likely due to the increased stability of the sinR mRNA) PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 199 PubMed

Biological materials

• Mutants:
  • 1S121 (tasA::spec), PubMed, available at BGSC
  • GP1571 (cat), available in Jörg Stülke's lab
  • GP1663 (deletion of yqhG-sinI-sinR-tasA), available in Jörg Stülke's lab
  • GP1672 (deletion of sinR-tasA::cat) PubMed, available in Jörg Stülke's lab
  • GP1586 (cat) in the NCIB3610 background, available in Jörg Stülke's lab
  • GP1819 (aphA3), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: