SwrAA/1

• Description: modulator of DegU activity, enhances sigD transcription, controls the number of flagellar basal bodies, inactive pseudogene in strain 168
  
  

Gene name	swrAA/1
Synonyms	yvzD, swrAA, ifm
Essential	no
Product	swarming motility protein
Function	control of DegU activity
Gene expression levels in SubtiExpress: swrAA/1
Interactions involving this protein in SubtInteract: SwrA
MW, pI	- , -
Gene length, protein length	336 bp, -
Immediate neighbours	minJ, swrAA/2
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biofilm formation, motility and chemotaxis, transcription factors and their control, pseudogenes

The gene

Basic information

• Locus tag: BSU35230

Phenotypes of a mutant

• loss of swarming motility PubMed

Database entries

• BsubCyc: BSU35230

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

• This protein is functional in undomesticated strains of B.subtilis but not in laboratory strains, such as 168, because of a frameshift mutation. Therefore laboratory strains of B.subtilis are unable to swarm.
• The frameshift in strain 168 is caused by a single insertion of an adenine in the codon for Tyr-12 which leads to the premature truncation of the protein in residue 13. In addition, the C-terminal section of swrAA was predicted to be an ORF (yvzD) by the genome project.
• Correction of sfp, epsC, swrAA, and degQ as well as introduction of rapP from a plasmid present in NCIB3610 results in biofilm formation in B. subtilis 168 PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • interacts with the N-terminal domain of DegU to control the activity of DegU PubMed
• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SwrA-DegU-P (N-terminal domain) PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU35230

• Structure:

• Swiss prot entry: O32266

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: swrAA-minJ PubMed

• Expression browser: swrAA/1 PubMed

• Sigma factor: SigD PubMed, SigA PubMed

• Regulation:

• Regulatory mechanism:
  • DegU-P: transcription activation (SigA promoter) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: