Difference between revisions of "RpoY"

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m (Jstuelk moved page YkzG to RpoY: 25092033)
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|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ykzG''
+
|''rpoY''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykzG ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
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|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[RNA polymerase]] activity
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[RNA polymerase]] activity
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14540 ykzG]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14540 rpoY]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 8 kDa, 4.605   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 8 kDa, 4.605   
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[ykzG]]-[[rnjA]]'' {{PubMed|24187087}}
+
* '''Operon:''' ''[[rpoY]]-[[rnjA]]'' {{PubMed|24187087}}
  
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ykzG_1524791_1525000_-1 ykzG] {{PubMed|22383849}}
+
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ykzG_1524791_1525000_-1 rpoY] {{PubMed|22383849}}
  
 
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|24187087}}
 
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|24187087}}

Revision as of 12:18, 6 August 2014

Gene name rpoY
Synonyms ykzG
Essential no
Product epsilon subunit of RNA polymerase
Function control of RNA polymerase activity
Gene expression levels in SubtiExpress: rpoY
MW, pI 8 kDa, 4.605
Gene length, protein length 207 bp, 69 aa
Immediate neighbours rnjA, ykrA
Sequences Protein DNA DNA_with_flanks
Genetic context
YkzG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkzG expression.png















Categories containing this gene/protein

transcription

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU14540

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0356 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • translation of YkzG and RnjA is coupled, and this coupling is required for efficient expression of RNase J1 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 443 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP420 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Andrew N Keller, Xiao Yang, Jana Wiedermannová, Olivier Delumeau, Libor Krásný, Peter J Lewis
ε, a new subunit of RNA polymerase found in gram-positive bacteria.
J Bacteriol: 2014, 196(20);3622-32
[PubMed:25092033] [WorldCat.org] [DOI] (I p)

Ailar Jamalli, Agnès Hébert, Léna Zig, Harald Putzer
Control of expression of the RNases J1 and J2 in Bacillus subtilis.
J Bacteriol: 2014, 196(2);318-24
[PubMed:24187087] [WorldCat.org] [DOI] (I p)

Geoff P Doherty, Mark J Fogg, Anthony J Wilkinson, Peter J Lewis
Small subunits of RNA polymerase: localization, levels and implications for core enzyme composition.
Microbiology (Reading): 2010, 156(Pt 12);3532-3543
[PubMed:20724389] [WorldCat.org] [DOI] (I p)