Pgk

• Description: phosphoglycerate kinase, glycolytic/ gluconeogenic enzyme, universally conserved protein
  
  

Gene name	pgk
Synonyms
Essential	No
Product	phosphoglycerate kinase
Function	enzyme in glycolysis/ gluconeogenesis
Gene expression levels in SubtiExpress: pgk
Metabolic function and regulation of this protein in SubtiPathways: pgk
MW, pI	42,0 kDa, 4.77
Gene length, protein length	1182 bp, 394 amino acids
Immediate neighbours	tpi, gapA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ATP synthesis, carbon core metabolism, phosphoproteins, universally conserved proteins, most abundant proteins

This gene is a member of the following regulons

CggR regulon

The gene

Basic information

• Locus tag: BSU33930

Phenotypes of a mutant

• essential PubMed, non-essential according to PubMed
• suppression of ftsZ(ts) mutation (reverted by addition of pyruvate) PubMed

Database entries

• BsubCyc: BSU33930

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 3-phospho-D-glycerate = ADP + 1,3-bisphosphoglycerate (according to Swiss-Prot)

• Protein family: phosphoglycerate kinase family (according to Swiss-Prot)
• Paralogous protein(s):

Extended information on the protein

• Kinetic information: Two Substrate Reversible Michaelis-Menten PubMed

• Domains:
  • nucleotide binding domain (ATP) (350–353)
  • 2x substrate binding domain (21–23), (59–62)

• Modification: phosphorylation on Ser-183 AND Thr-299 PubMed, PubMed

• Cofactors: Mg2+ or Mn2+ PubMed

• Effectors of protein activity:
  • Inhibited by Co2+, NDP and NMP PubMed

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot), Cytoplasm (Homogeneous) PubMed

Database entries

• BsubCyc: BSU33930

• Structure: 1PHP (from Geobacillus stearothermophilus)

• UniProt: P40924

• KEGG entry: [3]

• E.C. number: 2.7.2.3

Additional information

• extensive information on the structure and enzymatic properties of Pgk can be found at Proteopedia

Expression and regulation

• Operon:
  • cggR-gapA-pgk-tpiA-pgm-eno PubMed
  • pgk-tpiA-pgm-eno PubMed

• Expression browser: pgk PubMed

• Sigma factor:
  • cggR: SigA PubMed
  • pgk: SigA PubMed

• Regulation:
  • expression activated by glucose (3.3 fold) PubMed
  • cggR: induced by glycolytic substrates CggR PubMed
  • pgk: constitutive PubMed

• Regulatory mechanism: transcription repression by CggR PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 12006 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 10462 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 5859 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 2120 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2593 PubMed

Biological materials

• Mutant:
  • GP699 (pgk::cat), available in Jörg Stülke's lab, PubMedlab
  • GP707 (pgk::erm), available in Jörg Stülke's lab, PubMed

• Expression vector:
  • pGP1102 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
  • pGP95 (N-terminal Strep-tag, in pGP172), available in Jörg Stülke's lab
  • pGP91 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Jörg Stülke's lab
  • pGP1513 (expression in B. subtilis, in pBQ200), available in Jörg Stülke's lab

• lacZ fusion: pGP514 (in pAC6), a series of promoter deletion variants is also available in pAC6, available in Jörg Stülke's lab

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available iin Jörg Stülke's lab

• Antibody: