DnaK

• Description: class I heat-shock protein (molecular chaperone)
  
  

Gene name	dnaK
Synonyms
Essential	no
Product	class I heat-shock protein (molecular chaperone)
Function	protein quality control
Gene expression levels in SubtiExpress: dnaK
Interactions involving this protein in SubtInteract: DnaK
Metabolic function and regulation of this protein in SubtiPathways: dnaK
MW, pI	65 kDa, 4.571
Gene length, protein length	1833 bp, 611 aa
Immediate neighbours	surC, grpE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

chaperones/ protein folding, heat shock proteins, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

• Locus tag: BSU25470

Database entries

• BsubCyc: BSU25470

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: heat shock protein 70 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • DnaK-DnaJ

• Localization:
  • cytoplasm (according to Swiss-Prot)
  • membrane-proximal (Spotty) PubMed
  • recruited to the membrane after ethanol stress PubMed

Database entries

• BsubCyc: BSU25470

• Structure:

• UniProt: P17820

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV PubMed

• Expression browser: dnaK PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by heat shock (HrcA) PubMed

• Regulatory mechanism:
  • HrcA: transcription repression PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 5860 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 22949 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 8450 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 4859 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 2421 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage