NifS

• Description: cysteine desulfurase, required for NAD biosynthesis
  
  

Gene name	nifS
Synonyms	iscS
Essential	no
Product	cysteine desulfurase
Function	NAD biosynthesis
Gene expression levels in SubtiExpress: nifS
Metabolic function and regulation of this protein in SubtiPathways: NifS
MW, pI	43 kDa, 6.425
Gene length, protein length	1185 bp, 395 aa
Immediate neighbours	nadB, nadR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

NadR regulon

The gene

Basic information

• Locus tag: BSU27880

Database entries

• BsubCyc: BSU27880

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine (according to Swiss-Prot)

• Protein family: NifS/iscS subfamily (according to Swiss-Prot)

• Paralogous protein(s): YrvO, NifZ, SufS, YcbU

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU27880

• Structure:

• UniProt: P38033

• KEGG entry: [3]

• E.C. number: 2.8.1.7

Expression and regulation

• Operon: nifS-nadR PubMed

• Expression browser: nifS PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of nicotinic acid (NadR) PubMed

• Regulatory mechanism:
  • NadR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 363 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 799 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Katherine A Black, Patricia C Dos Santos
Shared-intermediates in the biosynthesis of thio-cofactors: Mechanism and functions of cysteine desulfurases and sulfur acceptors.
Biochim Biophys Acta: 2015, 1853(6);1470-80
[PubMed:25447671] [WorldCat.org] [DOI] (P p)

Original publications

Paola Rossolillo, Ilaria Marinoni, Elisa Galli, Anna Colosimo, Alessandra M Albertini
YrxA is the transcriptional regulator that represses de novo NAD biosynthesis in Bacillus subtilis.
J Bacteriol: 2005, 187(20);7155-60
[PubMed:16199587] [WorldCat.org] [DOI] (P p)

Joo-Heon Park, Pieter C Dorrestein, Huili Zhai, Cynthia Kinsland, Fred W McLafferty, Tadhg P Begley
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).
Biochemistry: 2003, 42(42);12430-8
[PubMed:14567704] [WorldCat.org] [DOI] (P p)

D Sun, P Setlow
Cloning, nucleotide sequence, and regulation of the Bacillus subtilis nadB gene and a nifS-like gene, both of which are essential for NAD biosynthesis.
J Bacteriol: 1993, 175(5);1423-32
[PubMed:8444804] [WorldCat.org] [DOI] (P p)