Difference between revisions of "CwlO"
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** the leader mRNA is subject to cleavage by [[rny|RNase Y]], this results in limited expression of ''[[cwlO]]'' {{PubMed|24163346}} | ** the leader mRNA is subject to cleavage by [[rny|RNase Y]], this results in limited expression of ''[[cwlO]]'' {{PubMed|24163346}} | ||
** there is a [[SigB]]-dependent anti-sense transcript which is expressed in the stationary phase {{PubMed|24163346}} | ** there is a [[SigB]]-dependent anti-sense transcript which is expressed in the stationary phase {{PubMed|24163346}} | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 207 {{PubMed|24696501}} | ||
+ | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 200 {{PubMed|24696501}} | ||
=Biological materials = | =Biological materials = |
Revision as of 09:27, 17 April 2014
- Description: D,L-endopeptidase-type autolysin, primary autolytic pathway for cell elongation
Gene name | cwlO |
Synonyms | yzkA, yvcE |
Essential | no |
Product | endopeptidase-type autolysin |
Function | cell wall synthesis, cell elongation |
Gene expression levels in SubtiExpress: cwlO | |
Interactions involving this protein in SubtInteract: CwlO | |
MW, pI | 50 kDa, 5.326 |
Gene length, protein length | 1419 bp, 473 aa |
Immediate neighbours | trxB, yvcD |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
cell wall synthesis, cell wall degradation/ turnover
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34800
Phenotypes of a mutant
- a cwlO lytE mutant is not viable PubMed
- shorter, fatter cells, this can be rescued by addition of Mg(2+) PubMed
Database entries
- BsubCyc: BSU34800
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: peptidase C40 family (according to Swiss-Prot)
- Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- BsubCyc: BSU34800
- Structure:
- UniProt: P40767
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Additional information:
- The mRNA has a long 5' leader region (187 N). This may indicate RNA-based regulation PubMed
- the leader mRNA is subject to cleavage by RNase Y, this results in limited expression of cwlO PubMed
- there is a SigB-dependent anti-sense transcript which is expressed in the stationary phase PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 207 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 200 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications