MhqE

• Description: dioxygenase/glyoxalase
  
  

Gene name	mhqE
Synonyms	yodE
Essential	no
Product	dioxygenase/glyoxalase
Function	resistance to methyl hydroquinone and catechol
Gene expression levels in SubtiExpress: mhqE
MW, pI	33 kDa, 5.041
Gene length, protein length	909 bp, 303 aa
Immediate neighbours	mhqD, yoyD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

resistance against oxidative and electrophile stress

This gene is a member of the following regulons

MhqR regulon

The gene

Basic information

• Locus tag: BSU19570

Database entries

• BsubCyc: BSU19570

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: extradiol ring-cleavage dioxygenase family (according to UniProt)

• Paralogous protein(s): MhqA, MhqO

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU19570

• Structure: 3OAJ (MhqO, 48% identical residues)

• UniProt: O34543

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mhqE-mhqD PubMed

• Expression browser: mhqE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by 2-methylhydroquinone (MhqR) PubMed

• Regulatory mechanism:
  • MhqR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Stefanie Töwe, Montira Leelakriangsak, Kazuo Kobayashi, Nguyen Van Duy, Michael Hecker, Peter Zuber, Haike Antelmann
The MarR-type repressor MhqR (YkvE) regulates multiple dioxygenases/glyoxalases and an azoreductase which confer resistance to 2-methylhydroquinone and catechol in Bacillus subtilis.
Mol Microbiol: 2007, 66(1);40-54
[PubMed:17725564] [WorldCat.org] [DOI] (P p)

Van Duy Nguyen, Carmen Wolf, Ulrike Mäder, Michael Lalk, Peter Langer, Ulrike Lindequist, Michael Hecker, Haike Antelmann
Transcriptome and proteome analyses in response to 2-methylhydroquinone and 6-brom-2-vinyl-chroman-4-on reveal different degradation systems involved in the catabolism of aromatic compounds in Bacillus subtilis.
Proteomics: 2007, 7(9);1391-408
[PubMed:17407181] [WorldCat.org] [DOI] (P p)