OdhB

• Description: 2-oxoglutarate dehydrogenase complex (dihydrolipoamide transsuccinylase, E2 subunit)
  
  

Gene name	odhB
Synonyms	citM
Essential	no PubMed
Product	2-oxoglutarate dehydrogenase complex (dihydrolipoamide transsuccinylase, E2 subunit)
Function	TCA cycle
Gene expression levels in SubtiExpress: odhB
Interactions involving this protein in SubtInteract: OdhB
Metabolic function and regulation of this protein in SubtiPathways: odhB
MW, pI	45 kDa, 4.859
Gene length, protein length	1251 bp, 417 aa
Immediate neighbours	yocS, odhA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, membrane proteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

• Locus tag: BSU19360

Database entries

• BsubCyc: BSU19360

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Succinyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-succinyldihydrolipoyl)lysine (according to Swiss-Prot)

• Protein family: sodium:bile acid symporter family (according to Swiss-Prot)

• Paralogous protein(s): AcoC, BkdB, PdhC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on several Arg residues PubMed

• Cofactors:
  • lipoic acid

• Effectors of protein activity:

• Interactions:
  • OdhA-OdhB-PdhD PubMed
  • OdhB-Icd PubMed

• Localization:
  • membrane associated PubMed

Database entries

• BsubCyc: BSU19360

• Structure: 1C4T (from Escherichia coli bl21, 57% identity, 78% similarity) PubMed

• UniProt: P16263

• KEGG entry: [3]

• E.C. number: 2.3.1.61

Additional information

• extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at Proteopedia

Expression and regulation

• Operon: odhA-odhB PubMed

• Expression browser: odhB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (2.4-fold) (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2581 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 42720 PubMed

Biological materials

• Mutant:
  • GP1276 (odhA-odhB::cat), available in Jörg Stülke's lab PubMed

• Expression vector:
  • pGP1145 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Stülke lab) PubMed

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab PubMed

• Antibody:

• FLAG-tag construct: GP1424 (spc, based on pGP1331), available in the Stülke lab