Difference between revisions of "DegU"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
−
* '''Description:''' [[two-component systems|two-component]] response regulator, regulation of degradative enzyme and genetic competence, involved in activation of capsule biosynthetic operon expression (together with [[SwrAA]]), non-phosphorylated DegU is required for swarming motility [http://www3.interscience.wiley.com/journal/122328658/abstract PubMed] <br/><br/>
+
* '''Description:''' [[two-component systems|two-component]] response regulator, regulation of degradative enzyme expression, [[genetic competence]], [[biofilm formation]], capsule biosynthesis (together with [[SwrAA]]), non-phosphorylated DegU is required for swarming motility <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
Line 12: Line 12:
 
|style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] response regulator
 
|style="background:#ABCDEF;" align="center"| '''Product''' || [[two-component systems|two-component]] response regulator
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of degradative enzyme and genetic competence
+
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of degradative enzymes, [[genetic competence]], [[biofilm formation]] and capsule synthesis
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35490 degU]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35490 degU]
Line 74: Line 74:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:''' transcription activator of degradative enzyme genes (''[[sacB]]'' and ''[[degQ]]'') and of ''[[comK]]'' expression
+
* '''Catalyzed reaction/ biological activity:'''  
 +
** transcription activator (see [[DegU regulon]])
  
 
* '''Protein family:'''
 
* '''Protein family:'''
Line 86: Line 87:
 
* '''[[Domains]]:'''  
 
* '''[[Domains]]:'''  
  
−
* '''Modification:''' phosphorylated by [[DegS]] on Asp-56 [http://www.ncbi.nlm.nih.gov/sites/entrez/1901568 PubMed], this modulates DNA-binding activity  
+
* '''Modification:'''  
 +
** phosphorylated by [[DegS]] on Asp-56 [http://www.ncbi.nlm.nih.gov/sites/entrez/1901568 PubMed], this modulates DNA-binding activity  
 +
** phosphorylation by [[DegS]] occurs in response to inhibition of flagellar rotation {{PubMed|23888912}}
  
 
* '''[[Cofactors]]:'''
 
* '''[[Cofactors]]:'''
Line 165: Line 168:
 
<pubmed>12471443,,</pubmed>
 
<pubmed>12471443,,</pubmed>
 
==Original Publications==
 
==Original Publications==
−
<pubmed>17850253,8878039, 14563871,1901568,1321152,1459944, 18194340 18978066 10908654,18502860,10094672,19389763, 20815827 21742882 18414485,19420703,2428811, 7746142, 18502860, 19389763, 19416356, 19734658 1688843 20070525 18197985 15598897 12950930 17590234 8955341 22496484 22745669 23123903 21965392 24123822 24296669</pubmed>
+
<pubmed>17850253,8878039, 14563871,1901568,1321152,1459944, 18194340 18978066 10908654,18502860,10094672,19389763, 20815827 21742882 18414485,19420703,2428811, 7746142, 18502860, 19389763, 19416356, 19734658 1688843 20070525 18197985 15598897 12950930 17590234 8955341 22496484 22745669 23123903 21965392 24123822 24296669 122328658 23888912</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:30, 16 February 2014

Gene name degU
Synonyms sacU, iep
Essential no
Product two-component response regulator
Function regulation of degradative enzymes, genetic competence, biofilm formation and capsule synthesis
Gene expression levels in SubtiExpress: degU
Interactions involving this protein in SubtInteract: DegU
Metabolic function and regulation of this protein in SubtiPathways:
Protein secretion
MW, pI 25 kDa, 5.602
Gene length, protein length 687 bp, 229 aa
Immediate neighbours yviA, degS
Sequences Protein DNA DNA_with_flanks
Genetic context
DegU context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DegU expression.png















Categories containing this gene/protein

biofilm formation, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, DegU regulon, TnrA regulon

The DegU regulon

The gene

Basic information

  • Locus tag: BSU35490

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • phosphorylated by DegS on Asp-56 PubMed, this modulates DNA-binding activity
    • phosphorylation by DegS occurs in response to inhibition of flagellar rotation PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information


Expression and regulation

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


The DegU regulon

Original Publications

Retrieved from "http://subtiwiki.uni-goettingen.de/wiki//index.php?title=DegU&oldid=197717"