GntR

• Description: transcriptional repressor of the gntR-gntK-gntP-gntZ operon
  
  

Gene name	gntR
Synonyms
Essential	no
Product	transcriptional repressor of the gluconate operon
Function	regulation of gluconate utilization
Gene expression levels in SubtiExpress: gntR
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	28 kDa, 5.26
Gene length, protein length	729 bp, 243 aa
Immediate neighbours	yxaA, gntK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control

This gene is a member of the following regulons

AbrB regulon, CcpA regulon, GntR regulon

The GntR regulon:

• gntR-gntK-gntP-gntZ

The gene

Basic information

• Locus tag: BSU40050

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: gluconate acts as molecular inducer

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P10585

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gntR-gntK-gntP-gntZ PubMed1PubMed2

• Expression browser: gntR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by gluconate (GntR) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • GntR: transcription repression PubMed
  • CcpA: transcription repression PubMed
  • AbrB: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Maria A Schumacher, Mareen Sprehe, Maike Bartholomae, Wolfgang Hillen, Richard G Brennan
Structures of carbon catabolite protein A-(HPr-Ser46-P) bound to diverse catabolite response element sites reveal the basis for high-affinity binding to degenerate DNA operators.
Nucleic Acids Res: 2011, 39(7);2931-42
[PubMed:21106498] [WorldCat.org] [DOI] (I p)

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)

Y Miwa, K Nagura, S Eguchi, H Fukuda, J Deutscher, Y Fujita
Catabolite repression of the Bacillus subtilis gnt operon exerted by two catabolite-responsive elements.
Mol Microbiol: 1997, 23(6);1203-13
[PubMed:9106211] [WorldCat.org] [DOI] (P p)

J Reizer, U Bergstedt, A Galinier, E Küster, M H Saier, W Hillen, M Steinmetz, J Deutscher
Catabolite repression resistance of gnt operon expression in Bacillus subtilis conferred by mutation of His-15, the site of phosphoenolpyruvate-dependent phosphorylation of the phosphocarrier protein HPr.
J Bacteriol: 1996, 178(18);5480-6
[PubMed:8808939] [WorldCat.org] [DOI] (P p)

K Yoshida, Y Miwa, H Ohmori, Y Fujita
Analysis of an insertional operator mutation (gntOi) that affects the expression level of the Bacillus subtilis gnt operon, and characterization of gntOi suppressor mutations.
Mol Gen Genet: 1995, 248(5);583-91
[PubMed:7476858] [WorldCat.org] [DOI] (P p)

Y Fujita, Y Miwa, A Galinier, J Deutscher
Specific recognition of the Bacillus subtilis gnt cis-acting catabolite-responsive element by a protein complex formed between CcpA and seryl-phosphorylated HPr.
Mol Microbiol: 1995, 17(5);953-60
[PubMed:8596444] [WorldCat.org] [DOI] (P p)

K Yoshida, H Ohmori, Y Miwa, Y Fujita
Bacillus subtilis gnt repressor mutants that diminish gluconate-binding ability.
J Bacteriol: 1995, 177(16);4813-6
[PubMed:7642511] [WorldCat.org] [DOI] (P p)

Y Fujita, Y Miwa
Catabolite repression of the Bacillus subtilis gnt operon mediated by the CcpA protein.
J Bacteriol: 1994, 176(2);511-3
[PubMed:8288545] [WorldCat.org] [DOI] (P p)

Y Miwa, Y Fujita
Promoter-independent catabolite repression of the Bacillus subtilis gnt operon.
J Biochem: 1993, 113(6);665-71
[PubMed:8370661] [WorldCat.org] [DOI] (P p)

K Yoshida, Y Fujita, A Sarai
Missense mutations in the Bacillus subtilis gnt repressor that diminish operator binding ability.
J Mol Biol: 1993, 231(2);167-74
[PubMed:8510140] [WorldCat.org] [DOI] (P p)

A Reizer, J Deutscher, M H Saier, J Reizer
Analysis of the gluconate (gnt) operon of Bacillus subtilis.
Mol Microbiol: 1991, 5(5);1081-9
[PubMed:1659648] [WorldCat.org] [DOI] (P p)

Y Miwa, Y Fujita
Determination of the cis sequence involved in catabolite repression of the Bacillus subtilis gnt operon; implication of a consensus sequence in catabolite repression in the genus Bacillus.
Nucleic Acids Res: 1990, 18(23);7049-53
[PubMed:2124676] [WorldCat.org] [DOI] (P p)

Y Fujita, T Fujita, Y Miwa
Evidence for posttranscriptional regulation of synthesis of the Bacillus subtilis Gnt repressor.
FEBS Lett: 1990, 267(1);71-4
[PubMed:2163901] [WorldCat.org] [DOI] (P p)

Y Fujita, Y Miwa
Identification of an operator sequence for the Bacillus subtilis gnt operon.
J Biol Chem: 1989, 264(7);4201-6
[PubMed:2492998] [WorldCat.org] (P p)

Y Miwa, Y Fujita
Purification and characterization of a repressor for the Bacillus subtilis gnt operon.
J Biol Chem: 1988, 263(26);13252-7
[PubMed:2843515] [WorldCat.org] (P p)

Y Fujita, T Fujita
The gluconate operon gnt of Bacillus subtilis encodes its own transcriptional negative regulator.
Proc Natl Acad Sci U S A: 1987, 84(13);4524-8
[PubMed:3037520] [WorldCat.org] [DOI] (P p)

Y Fujita, T Fujita, Y Miwa, J Nihashi, Y Aratani
Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis.
J Biol Chem: 1986, 261(29);13744-53
[PubMed:3020045] [WorldCat.org] (P p)

Y Fujita, T Fujita
Identification and nucleotide sequence of the promoter region of the Bacillus subtilis gluconate operon.
Nucleic Acids Res: 1986, 14(3);1237-52
[PubMed:2419835] [WorldCat.org] [DOI] (P p)

Y Fujita, J Nihashi, T Fujita
The characterization and cloning of a gluconate (gnt) operon of Bacillus subtilis.
J Gen Microbiol: 1986, 132(1);161-9
[PubMed:3011959] [WorldCat.org] [DOI] (P p)