Difference between revisions of "CwlO"
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| − | * '''Description:''' D,L-endopeptidase-type autolysin <br/><br/> | + | * '''Description:''' D,L-endopeptidase-type autolysin, primary autolytic pathway for cell elongation <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | a ''[[cwlO]] [[lytE]]'' mutant is not viable {{PubMed|17581128,22139507}} | + | * a ''[[cwlO]] [[lytE]]'' mutant is not viable {{PubMed|17581128,22139507}} |
| + | * shorter, fatter cells {{PubMed|xxx}} | ||
=== Database entries === | === Database entries === | ||
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| + | ** [[CwlO]] requires activation by [[FtsE]]-[[FtsX]] {{PubMed|xxx}} | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| − | |||
** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed] | ** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed] | ||
| − | ** localizes to the lateral sidewall of the cell (via the N-terminal domain) {{PubMed|22139507}} | + | ** localizes to the outer lateral sidewall of the cell (via the N-terminal domain) {{PubMed|22139507}} |
=== Database entries === | === Database entries === | ||
Revision as of 12:02, 16 July 2013
- Description: D,L-endopeptidase-type autolysin, primary autolytic pathway for cell elongation
| Gene name | cwlO |
| Synonyms | yzkA, yvcE |
| Essential | no |
| Product | endopeptidase-type autolysin |
| Function | cell wall synthesis, cell proliferation |
| Gene expression levels in SubtiExpress: cwlO | |
| MW, pI | 50 kDa, 5.326 |
| Gene length, protein length | 1419 bp, 473 aa |
| Immediate neighbours | trxB, yvcD |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell wall degradation/ turnover
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34800
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: peptidase C40 family (according to Swiss-Prot)
- Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity
Extended information on the protein
- Kinetic information:
- Domains:
- C-terminal D,L-endopeptidase domain PubMed
- Modification:
- Cofactor(s):
Database entries
- Structure:
- UniProt: P40767
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
- The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Additional reviews: PubMed
Original publications
Additional publications: PubMed
