Difference between revisions of "EpsC"
| Line 46: | Line 46: | ||
{{SubtiWiki regulon|[[AbrB regulon]]}}, | {{SubtiWiki regulon|[[AbrB regulon]]}}, | ||
{{SubtiWiki regulon|[[EAR riboswitch]]}}, | {{SubtiWiki regulon|[[EAR riboswitch]]}}, | ||
| + | {{SubtiWiki regulon|[[RemA regulon]]}}, | ||
{{SubtiWiki regulon|[[SinR regulon]]}} | {{SubtiWiki regulon|[[SinR regulon]]}} | ||
| + | |||
=The gene= | =The gene= | ||
| Line 115: | Line 117: | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | ** [[SinR]]: transcription | + | ** [[SinR]]: transcription anti-activation (prevents binding of [[RemA]]) {{PubMed|23646920}} |
| + | ** [[RemA]]: transcription activation {{PubMed|23646920}} | ||
** [[AbrB]]: transcription repression {{PubMed|20817675}} | ** [[AbrB]]: transcription repression {{PubMed|20817675}} | ||
| Line 151: | Line 154: | ||
<pubmed>20374491 20230605 </pubmed> | <pubmed>20374491 20230605 </pubmed> | ||
===Regulation of the ''eps'' operon=== | ===Regulation of the ''eps'' operon=== | ||
| − | <pubmed>15661000,16430695,18047568,18647168 20817675 21856853 21815947</pubmed> | + | <pubmed>15661000,16430695,18047568,18647168 20817675 21856853 21815947 23646920</pubmed> |
===Other original publications=== | ===Other original publications=== | ||
<pubmed> 21278284 </pubmed> | <pubmed> 21278284 </pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 15:24, 24 May 2013
- Description: putative UDP-sugar epimerase, may be involved in extracellular polysaccharide synthesis, this gene is inactive in B. subtilis 168
| Gene name | epsC |
| Synonyms | yveM |
| Essential | no |
| Product | unknown |
| Function | biofilm formation |
| Gene expression levels in SubtiExpress: epsC | |
| Regulation of this protein in SubtiPathways: Biofilm | |
| MW, pI | 66 kDa, 8.775 |
| Gene length, protein length | 1794 bp, 598 aa |
| Immediate neighbours | epsD, epsB |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biofilm formation, membrane proteins
This gene is a member of the following regulons
AbrB regulon, EAR riboswitch, RemA regulon, SinR regulon
The gene
Basic information
- Locus tag: BSU34350
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
- Correction of sfp, epsC, swrAA, and degQ as well as introduction of rapP from a plasmid present in NCIB3610 results in biofilm formation in B. subtilis 168 PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: polysaccharide synthase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P71052
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
- induction by sequestration of SinR by SinI or SlrA PubMed
- the EAR riboswitch (eps-associated RNA switch) located between epsB and epsC mediates processive antitermination and allows expression of the long eps operon PubMed
- the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO operon is not expressed in a ymdB mutant PubMed
- the amount of the mRNA is substantially decreased upon depletion of RNase Y (this is likely due to the increased stability of the sinR mRNA) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Richard Losick, Harvard Univ., Cambridge, USA homepage
Your additional remarks
References
Reviews
Original publications
The EAR RNA switch
Regulation of the eps operon
Other original publications
