Difference between revisions of "PonA"
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22320 ponA] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22320 ponA] | ||
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| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PonA PonA] | ||
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 99 kDa, 4.752 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 99 kDa, 4.752 | ||
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* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** [[EzrA]]-[[PonA]] {{PubMed|18363795}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
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<pubmed>21371139 </pubmed> | <pubmed>21371139 </pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>8606187,15262952,9721295,10322023,19192185,7814321,18363795,14731276, 21320184,18763711 19192185, 18179421 23531131 </pubmed> | + | <pubmed>8606187,15262952,9721295,10322023,19192185,7814321,18363795,14731276, 21320184,18763711 19192185, 18179421 23531131 18363795</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:00, 9 September 2013
- Description: penicillin-binding protein 1A/1B
| Gene name | ponA |
| Synonyms | |
| Essential | no |
| Product | penicillin-binding protein 1A/1B |
| Function | bifunctional glucosyltransferase/ transpeptidase |
| Gene expression levels in SubtiExpress: ponA | |
| Interactions involving this protein in SubtInteract: PonA | |
| MW, pI | 99 kDa, 4.752 |
| Gene length, protein length | 2742 bp, 914 aa |
| Immediate neighbours | recU, ypoC |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22320
Phenotypes of a mutant
- prevents bulging of the cells when grown at low Mg(2+) concentrations, suppresses the lethal effect of a mreB mutation PubMed
- deletion of ponA restores growth and normal shape of a yvcK mutant on gluconeogenic carbon sources PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P39793
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation: constitutive during vegetative growth PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
- Antibody:
Labs working on this gene/protein
Jeff Errington, Newcastle University, UK homepage
Your additional remarks
References
Reviews
Johann Mignolet, Patrick H Viollier
A sweet twist gets Bacillus into shape.
Mol Microbiol: 2011, 80(2);283-5
[PubMed:21371139]
[WorldCat.org]
[DOI]
(I p)
Original publications
