Ldh

• Description: L-lactate dehydrogenase
  
  

Gene name	ldh
Synonyms	lctE
Essential	no
Product	L-lactate dehydrogenase
Function	overflow metabolism, fermentation
Gene expression levels in SubtiExpress: ldh
Interactions involving this protein in SubtInteract: Ldh
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	34 kDa, 5.394
Gene length, protein length	963 bp, 321 aa
Immediate neighbours	amyE, lctP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

Rex regulon

The gene

Basic information

• Locus tag: BSU03050

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-lactate + NAD⁺ = pyruvate + NADH (according to Swiss-Prot)

• Protein family: LDH family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Tyr-224 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms tetramers PubMed

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:
  • 3PQE
  • A general discussion of Ldh structure

• UniProt: P13714

• KEGG entry: [3]

• E.C. number: 1.1.1.27

Expression and regulation

• Operon: ldh-lctP PubMed

• Expression browser: ldh PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced under anaerobic conditions (Rex) PubMed

• Regulatory mechanism:
  • Rex: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional references: PubMed

Shanshan Li, Di Huang, Yong Li, Jianping Wen, Xiaoqiang Jia
Rational improvement of the engineered isobutanol-producing Bacillus subtilis by elementary mode analysis.
Microb Cell Fact: 2012, 11;101
[PubMed:22862776] [WorldCat.org] [DOI] (I e)

Susana Romero, Enrique Merino, Francisco Bolívar, Guillermo Gosset, Alfredo Martinez
Metabolic engineering of Bacillus subtilis for ethanol production: lactate dehydrogenase plays a key role in fermentative metabolism.
Appl Environ Microbiol: 2007, 73(16);5190-8
[PubMed:17586670] [WorldCat.org] [DOI] (P p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Jonas T Larsson, Annika Rogstam, Claes von Wachenfeldt
Coordinated patterns of cytochrome bd and lactate dehydrogenase expression in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 10);3323-3335
[PubMed:16207915] [WorldCat.org] [DOI] (P p)