PtpZ

• Description: phosphotyrosine protein phosphatase, antagonist to PtkA
  
  

Gene name	ptpZ
Synonyms	ywqE
Essential	no
Product	phosphotyrosine protein phosphatase, antagonist to PtkA
Function	protein tyrosine dephosphorylation
Gene expression levels in SubtiExpress: ptpZ
Interactions involving this protein in SubtInteract: PtpZ
MW, pI	28 kDa, 6.66
Gene length, protein length	762 bp, 254 aa
Immediate neighbours	ugd, ptkA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biofilm formation, protein modification

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

• Locus tag: BSU36240

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate (according to Swiss-Prot), dephosphorylation of Ugd, TuaD and PtkA PubMed

• Protein family: cpsB/capC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3QY7 PubMed

• UniProt: P96717

• KEGG entry: [2]

• E.C. number: 3.1.3.48

Expression and regulation

• Operon: tkmA-ptkA-ptpZ-ugd PubMed

• Expression browser: ptpZ PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • DegU-P: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Hyoun Sook Kim, Sang Jae Lee, Hye Jin Yoon, Doo Ri An, Do Jin Kim, Soon-Jong Kim, Se Won Suh
Crystal structures of YwqE from Bacillus subtilis and CpsB from Streptococcus pneumoniae, unique metal-dependent tyrosine phosphatases.
J Struct Biol: 2011, 175(3);442-50
[PubMed:21605684] [WorldCat.org] [DOI] (I p)

Taryn B Kiley, Nicola R Stanley-Wall
Post-translational control of Bacillus subtilis biofilm formation mediated by tyrosine phosphorylation.
Mol Microbiol: 2010, 78(4);947-63
[PubMed:20815827] [WorldCat.org] [DOI] (I p)

Ivan Mijakovic, Lucia Musumeci, Lutz Tautz, Dina Petranovic, Robert A Edwards, Peter Ruhdal Jensen, Tomas Mustelin, Josef Deutscher, Nunzio Bottini
In vitro characterization of the Bacillus subtilis protein tyrosine phosphatase YwqE.
J Bacteriol: 2005, 187(10);3384-90
[PubMed:15866923] [WorldCat.org] [DOI] (P p)

Ivan Mijakovic, Sandrine Poncet, Grégory Boël, Alain Mazé, Sylvie Gillet, Emmanuel Jamet, Paulette Decottignies, Christophe Grangeasse, Patricia Doublet, Pierre Le Maréchal, Josef Deutscher
Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases.
EMBO J: 2003, 22(18);4709-18
[PubMed:12970183] [WorldCat.org] [DOI] (P p)