Difference between revisions of "SspE"
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|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA    | |style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA    | ||
|-  | |-  | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://  | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU08660 sspE]  | 
|-  | |-  | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''  | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''  | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fabL]]'', ''[[ygaB]]''  | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fabL]]'', ''[[ygaB]]''  | ||
|-  | |-  | ||
| − | |  | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU08660 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU08660 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU08660 Advanced_DNA]  | 
|-  | |-  | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:sspE_context.gif]]  | |colspan="2" | '''Genetic context''' <br/> [[Image:sspE_context.gif]]  | ||
Revision as of 12:24, 13 May 2013
-  Description: small acid-soluble spore protein (major gamma-type SASP) 
 
| Gene name | sspE | 
| Synonyms | |
| Essential | no | 
| Product | small acid-soluble spore protein (major gamma-type SASP) | 
| Function | protection of spore DNA | 
| Gene expression levels in SubtiExpress: sspE | |
|  Metabolic function and regulation of this protein in SubtiPathways:  Lipid synthesis  | |
| MW, pI | 9 kDa, 8.945 | 
| Gene length, protein length | 252 bp, 84 aa | 
| Immediate neighbours | fabL, ygaB | 
| Sequences | Protein DNA Advanced_DNA | 
 Genetic context  
  This image was kindly provided by SubtiList 
 | |
Expression at a glance   PubMed 
 | |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
SigG regulon, SpoVT regulon, YfhP regulon
The gene
Basic information
- Locus tag: BSU08660
 
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
 
- SubtiList entry: [2]
 
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
 
- Protein family: gamma-type SASP family (according to Swiss-Prot)
 
- Paralogous protein(s):
 
Extended information on the protein
- Kinetic information:
 
- Domains:
 
- Modification:
 
- Cofactor(s):
 
- Effectors of protein activity:
 
Database entries
- Structure:
 
- UniProt: P07784
 
- KEGG entry: [3]
 
- E.C. number:
 
Additional information
Expression and regulation
- Regulation: repressed by casamino acids PubMed
 
- Regulatory mechanism:
 
- Additional information:
 
Biological materials
- Mutant:
 
- Expression vector:
 
- lacZ fusion:
 
- GFP fusion:
 
- two-hybrid system:
 
- Antibody:
 
Labs working on this gene/protein
Your additional remarks
References
Sandra M Ruzal, Patricia L Bustos, Carmen Sánchez-Rivas  
"A la recherche" of functions for the spore protein SASP-E from Bacillus subtilis. 
J Microbiol Biotechnol: 2013, 23(1);15-21 
[PubMed:23314362]
  [WorldCat.org]
 [DOI]
 (I p)
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker  
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability. 
J Bacteriol: 2002, 184(15);4288-95 
[PubMed:12107147]
  [WorldCat.org]
 [DOI]
 (P p)
B Setlow, K A McGinnis, K Ragkousi, P Setlow  
Effects of major spore-specific DNA binding proteins on Bacillus subtilis sporulation and spore properties. 
J Bacteriol: 2000, 182(24);6906-12 
[PubMed:11092849]
  [WorldCat.org]
 [DOI]
 (P p)
Hiroki Yamamoto, Masao Mori, Junichi Sekiguchi  
Transcription of genes near the sspE locus of the Bacillus subtilis genome. 
Microbiology (Reading): 1999, 145 ( Pt 8);2171-2180 
[PubMed:10463184]
  [WorldCat.org]
 [DOI]
 (P p)
P Fajardo-Cavazos, F Tovar-Rojo, P Setlow  
Effect of promoter mutations and upstream deletions on the expression of genes coding for small, acid-soluble spore proteins of Bacillus subtilis. 
J Bacteriol: 1991, 173(6);2011-6 
[PubMed:1900507]
  [WorldCat.org]
 [DOI]
 (P p)
D X Sun, P Stragier, P Setlow  
Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis. 
Genes Dev: 1989, 3(2);141-9 
[PubMed:2497051]
  [WorldCat.org]
 [DOI]
 (P p)
J M Mason, P Fajardo-Cavazos, P Setlow  
Levels of mRNAs which code for small, acid-soluble spore proteins and their LacZ gene fusions in sporulating cells of Bacillus subtilis. 
Nucleic Acids Res: 1988, 16(14A);6567-83 
[PubMed:2456528]
  [WorldCat.org]
 [DOI]
 (P p)
R H Hackett, P Setlow  
Cloning, nucleotide sequencing, and genetic mapping of the gene for small, acid-soluble spore protein gamma of Bacillus subtilis. 
J Bacteriol: 1987, 169(5);1985-92 
[PubMed:3106326]
  [WorldCat.org]
 [DOI]
 (P p)

