Difference between revisions of "SkfB"
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|style="background:#ABCDEF;" align="center"|'''Function''' || maturation of spore killing factor | |style="background:#ABCDEF;" align="center"|'''Function''' || maturation of spore killing factor | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01920 skfB] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SkfB SkfB] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SkfB SkfB] | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[skfA]]'', ''[[skfC]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[skfA]]'', ''[[skfC]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01920 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01920 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01920 Advanced_DNA] |
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|colspan="2" | '''Genetic context''' <br/> [[Image:ybcP_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ybcP_context.gif]] | ||
Revision as of 12:01, 13 May 2013
- Description: thioether bond forming radical SAM enzyme, catalyzes the first step in the maturation of spore killing factor
| Gene name | skfB |
| Synonyms | ybcP, ybcQ |
| Essential | no |
| Product | thioether bond forming radical SAM enzyme |
| Function | maturation of spore killing factor |
| Gene expression levels in SubtiExpress: skfB | |
| Interactions involving this protein in SubtInteract: SkfB | |
| MW, pI | 45 kDa, 4.934 |
| Gene length, protein length | 1230 bp, 410 aa |
| Immediate neighbours | skfA, skfC |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
toxins, antitoxins and immunity against toxins
This gene is a member of the following regulons
AbrB regulon, PhoP regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU01920
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: moaA/nifB/pqqE family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): contains two [4Fe-4S] clusters PubMed
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O31423
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information: Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript. The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region. PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original Publications
Additional publications: PubMed
