Difference between revisions of "GlmM"
| Line 22: | Line 22: | ||
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1344 bp, 448 aa | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1344 bp, 448 aa | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cdaR]]'', ''[[glmS]]'' |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB11953]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB11953]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' | ||
| Line 108: | Line 108: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[ | + | * '''Operon:''' ''[[cdaA]]-[[cdaR]]-[[glmM]]-[[glmS]]'' {{PubMed|23192352}} |
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmM_198497_199843_1 glmM] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=glmM_198497_199843_1 glmM] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigA]] {{PubMed|22211522}} | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|22211522}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| Line 147: | Line 147: | ||
=References= | =References= | ||
'''Additional publications:''' {{PubMed|22211522}} | '''Additional publications:''' {{PubMed|22211522}} | ||
| − | <pubmed> 17726680, 17218307, 8550580 , 10231382 , 10671448 20097688 20525796 21685296 </pubmed> | + | <pubmed> 17726680, 17218307, 8550580 , 10231382 , 10671448 20097688 20525796 21685296 23192352</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:38, 30 November 2012
- Description: phosphoglucosamine mutase, required for cell wall synthesis
| Gene name | glmM |
| Synonyms | ybbT |
| Essential | yes PubMed |
| Product | phosphoglucosamine mutase, required for cell wall synthesis |
| Function | cell wall synthesis |
| Gene expression levels in SubtiExpress: glmM | |
| Metabolic function and regulation of this protein in SubtiPathways: Murein recycling | |
| MW, pI | 48 kDa, 4.677 |
| Gene length, protein length | 1344 bp, 448 aa |
| Immediate neighbours | cdaR, glmS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell wall synthesis, biosynthesis of cell wall components, essential genes, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU01770
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate (according to Swiss-Prot)
- Protein family: phosphohexose mutase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: O34824
- KEGG entry: [2]
- E.C. number: 5.4.2.10
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
Biological materials
- Mutant:
- Expression vector:
- pGP400: (expression in B. subtilis in pBQ200), available in Jörg Stülke's lab
- pGP1401: (expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
- pGP1402: (cloning vector for glmM (GlmM S100A) in E. coli, in pBlueskript KS), available in Jörg Stülke's lab
- pGP1403: (expression of GlmM (S100A) in B. subtilis in pBQ200), available in Jörg Stülke's lab
- pGP1405: (expression, purification of GlmM (S100A) in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
- lacZ fusion:
- GP1340 (cat) based on pAC6, available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
