Difference between revisions of "CitG"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' GP718 (spec), available in [[Stülke]] lab | + | * '''Mutant:''' |
| + | ** GP718 (spec), available in [[Jörg Stülke]]'s lab | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | ** pGP1122 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]]) (available in [[Stülke]] lab) | + | ** pGP1122 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]]) (available in [[Jörg Stülke]]'s lab) |
| − | * '''lacZ fusion:''' pGP387 (in [[pAC7]]), available in [[Stülke]] lab | + | * '''lacZ fusion:''' |
| + | ** pGP387 (in [[pAC7]]), available in [[Jörg Stülke]]'s lab | ||
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab | + | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab |
* '''Antibody:''' | * '''Antibody:''' | ||
| − | * '''FLAG-tag construct:''' GP1132 (spc, based on [[pGP1331]]), available in | + | * '''FLAG-tag construct:''' |
| + | ** GP1132 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab | ||
=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
Revision as of 16:10, 30 October 2012
- Description: fumarase
| Gene name | citG |
| Synonyms | |
| Essential | no |
| Product | fumarate hydratase |
| Function | TCA cycle |
| Gene expression levels in SubtiExpress: citG | |
| Interactions involving this protein in SubtInteract: CitG | |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 50 kDa, 5.475 |
| Gene length, protein length | 1386 bp, 462 aa |
| Immediate neighbours | yuxN, yvzF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU33040
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate = fumarate + H2O (according to Swiss-Prot)
- Protein family: Fumarase subfamily (according to Swiss-Prot)
- Paralogous protein(s): AnsB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: P07343
- KEGG entry: [3]
- E.C. number: 4.2.1.2
Additional information
Expression and regulation
- Regulation: constitutive
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP718 (spec), available in Jörg Stülke's lab
- Expression vector:
- pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab)
- lacZ fusion:
- pGP387 (in pAC7), available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
- FLAG-tag construct:
- GP1132 (spc, based on pGP1331), available in Jörg Stülke's lab
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
