LytE

• Description: cell wall hydrolase (major autolysin), D,L-endopeptidase-type autolysin
  
  

Gene name	lytE
Synonyms	papQ, cwlF
Essential	no
Product	cell wall hydrolase (major autolysin),endopeptidase-type autolysin
Function	major autolysin, cell separation, cell proliferation
Gene expression levels in SubtiExpress: lytE
MW, pI	37 kDa, 10.713
Gene length, protein length	1029 bp, 343 aa
Immediate neighbours	phoA, citR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

SigH regulon, SigI regulon, Spo0A regulon, WalR regulon

The gene

Basic information

• Locus tag: BSU09420

Phenotypes of a mutant

• a cwlO lytE mutant is not viable PubMed
• growth defect at high temperature PubMed
• inactivation of lytE strongly restores beta-lactam resistance in a sigM mutant by delaying cell lysis PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nlpC/p60 family (according to Swiss-Prot)

• Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

• Kinetic information:

• Domains:
  • C-terminal D,L-endopeptidase domain PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • MreBH-LytE along the cylindrical part of the cell wall PubMed
  • FtsZ-LytE at the division septum PubMed
  • PbpB-LytE at the division septum PubMed

• Localization:
  • secreted (according to Swiss-Prot)
  • binds the cell wall PubMed
  • localizes to cell septa, poles and lateral sidewall of the cell (via the N-terminal domain) PubMed

Database entries

• Structure:

• UniProt: P54421

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lytE PubMed

• Expression browser: lytE PubMed

• Sigma factor: SigA PubMed, SigH PubMed, SigI PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • induced at high temperature (SigI) PubMed
• Regulatory mechanism:
  • WalR: transcription activation PubMed
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A790 ( lytE::cat), PubMed, available at BGSC
  • 1A792 ( lytE::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed