MleN

• Description: malate-H+/Na+-lactate antiporter
  
  

Gene name	mleN
Synonyms	yqkI
Essential	no
Product	malate-H+/Na+-lactate antiporter
Function	malate uptake
Gene expression levels in SubtiExpress: mleN
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	50 kDa, 7.249
Gene length, protein length	1404 bp, 468 aa
Immediate neighbours	mleA, ansB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

AnsR regulon, CcpA regulon

The gene

Basic information

• Locus tag: BSU23560

Phenotypes of a mutant

abrupt arrest in the mid-logarithmic phase of growth on malate when low concentrations of protonophore were present PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nhaC Na(+)/H(+) antiporter family (according to Swiss-Prot)

• Paralogous protein(s): NhaC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane associated PubMed

Database entries

• Structure:

• UniProt: P54571

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • ansA-ansB-mleN-mleA PubMed
  • mleN-mleA PubMed

• Expression browser: mleN PubMed

• Sigma factor:
  • ansA: SigA PubMed
  • mleN: SigA PubMed

• Regulation:
  • ansA: expressed in the presence of asparagine (AnsR) PubMed
  • mleN: repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • AnsR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:GP1460 (mleN::spc), available in Stülke lab

• Expression vector:

• lacZ fusion: pGP388 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody: