Difference between revisions of "YfmL"
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* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | + | ** for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [[SPINE]], expression from the native chromomsomal site: GP1069 (spc), available in [[Jörg Stülke]]'s lab | |
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
| − | * '''GFP fusion:''' B. subtilis GP1082 yfmL-yfp spc, available in [[Stülke]] lab | + | * '''GFP fusion:''' |
| + | ** B. subtilis GP1082 yfmL-yfp spc, available in [[Jörg Stülke]]'s lab | ||
| − | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab | + | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab |
| − | * '''FLAG-tag construct:''' GP1066 (spc, based on [[pGP1331]]), available in | + | * '''FLAG-tag construct:''' |
| + | ** GP1066 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s | ||
* '''Antibody:''' | * '''Antibody:''' | ||
Revision as of 12:39, 20 September 2012
- Description: DEAD-box RNA helicase
| Gene name | yfmL |
| Synonyms | |
| Essential | no |
| Product | DEAD-box RNA helicase |
| Function | RNA helicase |
| Gene expression levels in SubtiExpress: yfmL | |
| MW, pI | 42 kDa, 9.602 |
| Gene length, protein length | 1128 bp, 376 aa |
| Immediate neighbours | yfmM, yfmK |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU07430
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: RNA helicase
- Protein family: helicase C-terminal domain (according to Swiss-Prot) DEAD-box RNA helicase
- Paralogous protein(s): CshA, CshB, DeaD
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O34750
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: yfmL (according to DBTBS)
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1053 (erm), available in the Stülke lab
- Expression vector:
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1069 (spc), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- B. subtilis GP1082 yfmL-yfp spc, available in Jörg Stülke's lab
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- FLAG-tag construct:
- GP1066 (spc, based on pGP1331), available in Jörg Stülke's
- Antibody:
