ThyB

• Description: thymidylate synthase B
  
  

Gene name	thyB
Synonyms
Essential	no
Product	thymidylate synthase B
Function	biosynthesis of thymidine nucleotides
Interactions involving this protein in SubtInteract: ThyB
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation)
MW, pI	30 kDa, 6.272
Gene length, protein length	792 bp, 264 aa
Immediate neighbours	dfrA, ypjQ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, phosphoproteins

The gene

Basic information

• Locus tag: BSU21820

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-102 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DfrA-ThyB

• Localization:

Database entries

• Structure:

• UniProt: P11044

• KEGG entry: [2]

• E.C. number: 2.1.1.45

Expression and regulation

• Operon: thyB-dfrA PubMed

• Expression browser: thyB PubMed

• Sigma factor:

• Regulation:
  • weak induction in the presence of glucose (CcpN) PubMed

• Regulatory mechanism:
  • CcpN: weak transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

Rita A Eckart, Sabine Brantl, Andreas Licht
Search for additional targets of the transcriptional regulator CcpN from Bacillus subtilis.
FEMS Microbiol Lett: 2009, 299(2);223-31
[PubMed:19732150] [WorldCat.org] [DOI] (I p)

M Iwakura, M Kawata, K Tsuda, T Tanaka
Nucleotide sequence of the thymidylate synthase B and dihydrofolate reductase genes contained in one Bacillus subtilis operon.
Gene: 1988, 64(1);9-20
[PubMed:2840350] [WorldCat.org] [DOI] (P p)