Btr

• Description: transcription activator (AraC family DNA-binding domain fused to FeuA-like substrate-binding domain), regulation of the feuA-feuB-feuC-ybbA operon
  
  

Gene name	btr
Synonyms	ybbB, yzbC
Essential	no
Product	transcription activator (AraC family)
Function	regulation of iron acquisition
Metabolic function and regulation of this protein in SubtiPathways: Stress, Metal ion homeostasis
MW, pI	60 kDa, 6.433
Gene length, protein length	1587 bp, 529 aa
Immediate neighbours	feuA, ybbC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

acquisition of iron, iron metabolism, transcription factors and their control

This gene is a member of the following regulons

Fur regulon

The Btr regulon:

• feuA-feuB-feuC-ybbA

The gene

Basic information

• Locus tag: BSU01640

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binding of a direct repeat in the feuA promoter region and transcription activation of the feuA-feuB-feuC-ybbA operon in the presence of bacillibactin or enterobactin PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): enterobactin, bacillibactin (co-activators) PubMed

• Effectors of protein activity: enterobactin, bacillibactin (co-activators) PubMed

• Interactions:
  • Btr-RNA polymerase (perhaps SigA) PubMed

• Localization:

Database entries

• Structure:

• UniProt: P40408

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: btr PubMed

• Sigma factor:

• Regulation:
  • expressed under conditions of iron starvation (Fur) PubMed

• Regulatory mechanism:
  • Fur: transcription repression PubMed

• Additional information:
  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: