Difference between revisions of "LytE"
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| − | * '''Description:''' cell wall hydrolase (major autolysin),endopeptidase-type autolysin <br/><br/> | + | * '''Description:''' cell wall hydrolase (major autolysin), D,L-endopeptidase-type autolysin <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Product''' || cell wall hydrolase (major autolysin),endopeptidase-type autolysin | |style="background:#ABCDEF;" align="center"| '''Product''' || cell wall hydrolase (major autolysin),endopeptidase-type autolysin | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || major autolysin, cell separation, cell | + | |style="background:#ABCDEF;" align="center"|'''Function''' || major autolysin, cell separation, cell proliferation |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 10.713 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 10.713 | ||
| Line 49: | Line 49: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | ** a ''[[cwlO]] [[lytE]]'' mutant is not viable {{PubMed|17581128}} | + | ** a ''[[cwlO]] [[lytE]]'' mutant is not viable {{PubMed|17581128,22139507}} |
** growth defect at high temperature {{PubMed|21541672}} | ** growth defect at high temperature {{PubMed|21541672}} | ||
| Line 76: | Line 76: | ||
* '''Domains:''' | * '''Domains:''' | ||
| + | ** C-terminal D,L-endopeptidase domain {{PubMed|22139507}} | ||
* '''Modification:''' | * '''Modification:''' | ||
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** [[MreBH]]-[[LytE]] {{PubMed|16950129}} | ** [[MreBH]]-[[LytE]] {{PubMed|16950129}} | ||
| − | * '''[[Localization]]:''' secreted (according to Swiss-Prot) | + | * '''[[Localization]]:''' |
| + | ** secreted (according to Swiss-Prot) | ||
| + | ** binds the cell wall {{PubMed|21261835}} | ||
| + | ** localizes to cell septa, poles and lateral sidewall of the cell (via the N-terminal domain) {{PubMed|22139507}} | ||
=== Database entries === | === Database entries === | ||
Revision as of 11:58, 8 December 2011
- Description: cell wall hydrolase (major autolysin), D,L-endopeptidase-type autolysin
| Gene name | lytE |
| Synonyms | papQ, cwlF |
| Essential | no |
| Product | cell wall hydrolase (major autolysin),endopeptidase-type autolysin |
| Function | major autolysin, cell separation, cell proliferation |
| MW, pI | 37 kDa, 10.713 |
| Gene length, protein length | 1029 bp, 343 aa |
| Immediate neighbours | phoA, citR |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
cell wall degradation/ turnover
This gene is a member of the following regulons
SigH regulon, SigI regulon, Spo0A regulon, WalR regulon
The gene
Basic information
- Locus tag: BSU09420
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: nlpC/p60 family (according to Swiss-Prot)
- Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity
Extended information on the protein
- Kinetic information:
- Domains:
- C-terminal D,L-endopeptidase domain PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P54421
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: lytE PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
Chi-Ling Tseng, Jung-Tze Chen, Ju-Hui Lin, Wan-Zhen Huang, Gwo-Chyuan Shaw
Genetic evidence for involvement of the alternative sigma factor SigI in controlling expression of the cell wall hydrolase gene lytE and contribution of LytE to heat survival of Bacillus subtilis.
Arch Microbiol: 2011, 193(9);677-85
[PubMed:21541672]
[WorldCat.org]
[DOI]
(I p)
Paola Bisicchia, David Noone, Efthimia Lioliou, Alistair Howell, Sarah Quigley, Thomas Jensen, Hanne Jarmer, Kevin M Devine
The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis.
Mol Microbiol: 2007, 65(1);180-200
[PubMed:17581128]
[WorldCat.org]
[DOI]
(P p)
Rut Carballido-López, Alex Formstone, Ying Li, S Dusko Ehrlich, Philippe Noirot, Jeff Errington
Actin homolog MreBH governs cell morphogenesis by localization of the cell wall hydrolase LytE.
Dev Cell: 2006, 11(3);399-409
[PubMed:16950129]
[WorldCat.org]
[DOI]
(P p)
Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647]
[WorldCat.org]
[DOI]
(P p)
Hiroki Yamamoto, Shin-ichirou Kurosawa, Junichi Sekiguchi
Localization of the vegetative cell wall hydrolases LytC, LytE, and LytF on the Bacillus subtilis cell surface and stability of these enzymes to cell wall-bound or extracellular proteases.
J Bacteriol: 2003, 185(22);6666-77
[PubMed:14594841]
[WorldCat.org]
[DOI]
(P p)
R Ohnishi, S Ishikawa, J Sekiguchi
Peptidoglycan hydrolase LytF plays a role in cell separation with CwlF during vegetative growth of Bacillus subtilis.
J Bacteriol: 1999, 181(10);3178-84
[PubMed:10322020]
[WorldCat.org]
[DOI]
(P p)
S Ishikawa, Y Hara, R Ohnishi, J Sekiguchi
Regulation of a new cell wall hydrolase gene, cwlF, which affects cell separation in Bacillus subtilis.
J Bacteriol: 1998, 180(9);2549-55
[PubMed:9573210]
[WorldCat.org]
[DOI]
(P p)
P Margot, M Wahlen, A Gholamhoseinian, P Piggot, D Karamata
The lytE gene of Bacillus subtilis 168 encodes a cell wall hydrolase.
J Bacteriol: 1998, 180(3);749-52
[PubMed:9457885]
[WorldCat.org]
[DOI]
(P p)
