Difference between revisions of "Scr"
| Line 8: | Line 8: | ||
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || | + | |style="background:#ABCDEF;" align="center"| '''Essential''' || yes |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || small cytoplasmic RNA (scRNA, 4.5S RNA), | |style="background:#ABCDEF;" align="center"| '''Product''' || small cytoplasmic RNA (scRNA, 4.5S RNA), | ||
| Line 46: | Line 46: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | + | * the gene is essential {{PubMed|7506707}} | |
=== Database entries === | === Database entries === | ||
| Line 107: | Line 107: | ||
=References= | =References= | ||
| − | <pubmed>9346318,2468993,17576666, </pubmed> | + | <pubmed>9346318,2468993,17576666, 1372600,7506707 1383945 7538759 10224127 9677377 8662730 10222262</pubmed> |
[[Category:small RNA]] | [[Category:small RNA]] | ||
Revision as of 15:40, 3 April 2012
- Description: 4.5 S RNA
| Gene name | scr |
| Synonyms | |
| Essential | yes |
| Product | small cytoplasmic RNA (scRNA, 4.5S RNA),
signal recognition particle-like (SRP) component |
| Function | presecretory protein translocation |
| Interactions involving this RNA in SubtInteract: Scr | |
| Immediate neighbours | yaaJ, dnaX |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag:
Phenotypes of a mutant
- the gene is essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
- Structure:
Additional information
The RNA
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
Extended information on the RNA
- Kinetic information
Database entries
- Structure:
Additional information
Expression and regulation
- Sigma factor: SigA (both promoters)
- Regulation:
- Regulatory mechanism:
- Additional information: transcribed as 354 nt precursor, cleaved at the 5' end by RNase III and at the 3' site by RNase J1 to yield a 275 nt molecule, final trimming to 271 nt sc RNA by RNase PH PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
Labs working on this gene/protein
David Bechhofer, Mount Sinai School, New York, USA Homepage
Your additional remarks
References
Shiyi Yao, Joshua B Blaustein, David H Bechhofer
Processing of Bacillus subtilis small cytoplasmic RNA: evidence for an additional endonuclease cleavage site.
Nucleic Acids Res: 2007, 35(13);4464-73
[PubMed:17576666]
[WorldCat.org]
[DOI]
(I p)
K Nakamura, S Yahagi, T Yamazaki, K Yamane
Bacillus subtilis histone-like protein, HBsu, is an integral component of a SRP-like particle that can bind the Alu domain of small cytoplasmic RNA.
J Biol Chem: 1999, 274(19);13569-76
[PubMed:10224127]
[WorldCat.org]
[DOI]
(P p)
T Yamazaki, S Yahagi, K Nakamura, K Yamane
Depletion of Bacillus subtilis histone-like protein, HBsu, causes defective protein translocation and induces upregulation of small cytoplasmic RNA.
Biochem Biophys Res Commun: 1999, 258(1);211-4
[PubMed:10222262]
[WorldCat.org]
[DOI]
(P p)
A Oguro, H Kakeshita, K Nakamura, K Yamane, W Wang, D H Bechhofer
Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor.
J Biol Chem: 1998, 273(31);19542-7
[PubMed:9677377]
[WorldCat.org]
[DOI]
(P p)
H Takamatsu, K Bunai, T Horinaka, A Oguro, K Nakamura, K Watabe, K Yamane
Identification of a region required for binding to presecretory protein in Bacillus subtilis Ffh, a homologue of the 54-kDa subunit of mammalian signal recognition particle.
Eur J Biochem: 1997, 248(2);575-82
[PubMed:9346318]
[WorldCat.org]
[DOI]
(P p)
K Kurita, K Honda, S Suzuma, H Takamatsu, K Nakamura, K Yamane
Identification of a region of Bacillus subtilis Ffh, a homologue of mammalian SRP54 protein, that is essential for binding to small cytoplasmic RNA.
J Biol Chem: 1996, 271(22);13140-6
[PubMed:8662730]
[WorldCat.org]
[DOI]
(P p)
T Shibata, Y Nakamura, K Nakamura, K Yamane
Depletion of small cytoplasmic RNA confers fusidic-acid resistance on Bacillus subtilis.
Biochem Biophys Res Commun: 1995, 210(2);317-23
[PubMed:7538759]
[WorldCat.org]
[DOI]
(P p)
M Nishiguchi, K Honda, R Amikura, K Nakamura, K Yamane
Structural requirements of Bacillus subtilis small cytoplasmic RNA for cell growth, sporulation, and extracellular enzyme production.
J Bacteriol: 1994, 176(1);157-65
[PubMed:7506707]
[WorldCat.org]
[DOI]
(P p)
K Nakamura, M Minemura, M Nishiguchi, K Honda, A Nakamura, K Yamane
Conserved residues and secondary structure found in small cytoplasmic RNAs from thirteen Bacillus species.
Nucleic Acids Res: 1992, 20(19);5227-8
[PubMed:1383945]
[WorldCat.org]
[DOI]
(P p)
K Nakamura, Y Imai, A Nakamura, K Yamane
Small cytoplasmic RNA of Bacillus subtilis: functional relationship with human signal recognition particle 7S RNA and Escherichia coli 4.5S RNA.
J Bacteriol: 1992, 174(7);2185-92
[PubMed:1372600]
[WorldCat.org]
[DOI]
(P p)
J C Struck, R K Hartmann, H Y Toschka, V A Erdmann
Transcription and processing of Bacillus subtilis small cytoplasmic RNA.
Mol Gen Genet: 1989, 215(3);478-82
[PubMed:2468993]
[WorldCat.org]
[DOI]
(P p)
