Difference between revisions of "CapE"
(→Categories containing this gene/protein) |
|||
Line 32: | Line 32: | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
− | {{SubtiWiki category|[[ | + | {{SubtiWiki category|[[capsule bioynthesis and degradation]]}} |
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = |
Revision as of 11:57, 8 November 2011
- Description: stimulates poly-gamma-glutamate production in the presence of zinc, also involved in extra-chromosomal DNA maintenance
Gene name | capE |
Synonyms | ywtC, pgsE |
Essential | no |
Product | unknown |
Function | capsule synthesis |
MW, pI | 6 kDa, 6.487 |
Gene length, protein length | 165 bp, 55 aa |
Immediate neighbours | pgdS, capA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
Categories containing this gene/protein
capsule bioynthesis and degradation
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU35870
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: stimulates poly-gamma-glutamate production in the presence of zinc PubMed, also involved in extra-chromosomal DNA maintenance PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P96739
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information: The operon is not expressed in the lab strain 168 due to lack of expression of DegQ resulting in inactivity of DegS and DegU PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
Daisuke Yamashiro, Yutaka Minouchi, Makoto Ashiuchi
Moonlighting role of a poly-gamma-glutamate synthetase component from Bacillus subtilis: insight into novel extrachromosomal DNA maintenance.
Appl Environ Microbiol: 2011, 77(8);2796-8
[PubMed:21357437]
[WorldCat.org]
[DOI]
(I p)
Tohru Kamei, Daisuke Yamashiro, Terumi Horiuchii, Yutaka Minouchi, Makoto Ashiuchi
Identification and biochemical characterization of membranous short-chain polyglutamate from Bacillus subtilis.
Chem Biodivers: 2010, 7(6);1563-72
[PubMed:20564574]
[WorldCat.org]
[DOI]
(I p)
Chuan-Mei Yeh, Jyh-Perng Wang, Shih-Ching Lo, Wen-Chia Chan, Ming-Yi Lin
Chromosomal integration of a synthetic expression control sequence achieves poly-gamma-glutamate production in a Bacillus subtilis strain.
Biotechnol Prog: 2010, 26(4);1001-7
[PubMed:20564357]
[WorldCat.org]
[DOI]
(I p)
Keitarou Kimura, Lam-Son Phan Tran, Thi-Huyen Do, Yoshifumi Itoh
Expression of the pgsB encoding the poly-gamma-DL-glutamate synthetase of Bacillus subtilis (natto).
Biosci Biotechnol Biochem: 2009, 73(5);1149-55
[PubMed:19420703]
[WorldCat.org]
[DOI]
(I p)
Thomas Candela, Michèle Mock, Agnès Fouet
CapE, a 47-amino-acid peptide, is necessary for Bacillus anthracis polyglutamate capsule synthesis.
J Bacteriol: 2005, 187(22);7765-72
[PubMed:16267300]
[WorldCat.org]
[DOI]
(P p)
Yuji Urushibata, Shinji Tokuyama, Yasutaka Tahara
Difference in transcription levels of cap genes for gamma-polyglutamic acid production between Bacillus subtilis IFO 16449 and Marburg 168.
J Biosci Bioeng: 2002, 93(2);252-4
[PubMed:16233197]
[WorldCat.org]
[DOI]
(P p)