InfC

• Description: translation initiation factor IF-3
  
  

Gene name	infC
Synonyms
Essential	yes PubMed
Product	initiation factor IF-3
Function	translation
MW, pI	19 kDa, 10.342
Gene length, protein length	519 bp, 173 aa
Immediate neighbours	rpmI, yscA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

RplT regulon, stringent response

The gene

Basic information

• Locus tag: BSU28870

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: IF-3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1TIF (N-terminal domain, Geobacillus stearothermophilus), 1TIG (C-terminal domain, Geobacillus stearothermophilus)

• UniProt: P55872

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: infC-rpmI-rplT-ysdA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:
  • binding of RplT to a RNA switch in the infC leader region causes transcription termination PubMed

• Additional information:
  • expression of IF3 is uncoupled from that of L35 and L20 by RNase Y-dependent processing PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Patrice Bruscella, Karen Shahbabian, Soumaya Laalami, Harald Putzer
RNase Y is responsible for uncoupling the expression of translation factor IF3 from that of the ribosomal proteins L35 and L20 in Bacillus subtilis.
Mol Microbiol: 2011, 81(6);1526-41
[PubMed:21843271] [WorldCat.org] [DOI] (I p)

Nasslie Choonee, Sergine Even, Lena Zig, Harald Putzer
Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism.
Nucleic Acids Res: 2007, 35(5);1578-88
[PubMed:17289755] [WorldCat.org] [DOI] (I p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)