ManP

• Description: trigger enzyme: mannose-specific phosphotransferase system, EIIBCA of the PTS
  
  

Gene name	manP
Synonyms	yjdD
Essential	no
Product	trigger enzyme: mannose-specific phosphotransferase system, EIIBCA
Function	mannose uptake and phosphorylation, control of ManR activity
Interactions involving this protein in SubtInteract: ManP
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	61 kDa, 6.715
Gene length, protein length	1767 bp, 589 aa
Immediate neighbours	manR, manA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins

This gene is a member of the following regulons

ManR regulon

The gene

Basic information

• Locus tag: BSU12010

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transport and concomitant phosphorylation of mannose

• Protein family: PTS permease, fructose/ mannitol permease (Fru) family PubMed

• Paralogous protein(s): FruA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-365 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ManP-ManR, ManP-HPr

• Localization: membrane

Database entries

• Structure: 2R48 (IIB domain)

• UniProt: O31645

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: manP-manA-yjdF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by mannose (ManR) PubMed
  • repressed by glucose (ManR) PubMed

• Regulatory mechanism:
  • ManR: transcription activation in the presence of mannose and absence of glucose PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tianqi Sun, Josef Altenbuchner
Characterization of a mannose utilization system in Bacillus subtilis.
J Bacteriol: 2010, 192(8);2128-39
[PubMed:20139185] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)