ComGC

• Description: DNA uptake, major component of the pseudopilus
  
  

Gene name	comGC
Synonyms
Essential	no
Product	major pseudopilin
Function	genetic competence
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	10 kDa, 6.325
Gene length, protein length	294 bp, 98 aa
Immediate neighbours	comGD, comGB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

genetic competence, membrane proteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU24710

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: comGC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ComGB-ComGC PubMed
  • ComC-ComGC PubMed
  • forms large complexes (the pseudopilus) PubMed

• Localization:
  • cell surface PubMed
  • localizes to the cell membrane and the cytoplasm PubMed
  • localizes to one single cell pole in the majority of the cells PubMed

= Database entries

• Structure:

• UniProt: P25955

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: comGA-comGB-comGC-comGD-comGE-comGF-comGG-yqzE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159] [WorldCat.org] [DOI] (P p)