Difference between revisions of "ProH"
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|style="background:#ABCDEF;" align="center"| '''Product''' || pyrroline-5-carboxylate reductase | |style="background:#ABCDEF;" align="center"| '''Product''' || pyrroline-5-carboxylate reductase | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || osmoadaptive de novo production of proline |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/proline.html Pro], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/proline.html Pro], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]''' | ||
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* '''Operon:''' ''[[proH]]-[[proJ]]'' {{PubMed|21296969}} | * '''Operon:''' ''[[proH]]-[[proJ]]'' {{PubMed|21296969}} | ||
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|21784929}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** expressed under conditions of osmotic stress | + | ** expressed under conditions of osmotic stress {{PubMed|21784929}} |
** induced by cold stress {{PubMed|21296969}} | ** induced by cold stress {{PubMed|21296969}} | ||
| Line 130: | Line 130: | ||
=References= | =References= | ||
| − | <pubmed>,11418582, 21296969</pubmed> | + | <pubmed> 21784929,11418582, 21296969</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:37, 26 July 2011
- Description: pyrroline-5-carboxylate reductase
| Gene name | proH |
| Synonyms | yoxE |
| Essential | no |
| Product | pyrroline-5-carboxylate reductase |
| Function | osmoadaptive de novo production of proline |
| Metabolic function and regulation of this protein in SubtiPathways: Pro, Stress | |
| MW, pI | 31 kDa, 7.754 |
| Gene length, protein length | 891 bp, 297 aa |
| Immediate neighbours | proJ, rtp |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU18480
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H (according to Swiss-Prot)
- Protein family: glutamate 5-kinase family (according to Swiss-Prot)
- Paralogous protein(s): ProI
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P14383
- KEGG entry: [2]
- E.C. number: 1.5.1.2
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Jeanette Brill, Tamara Hoffmann, Monika Bleisteiner, Erhard Bremer
Osmotically controlled synthesis of the compatible solute proline is critical for cellular defense of Bacillus subtilis against high osmolarity.
J Bacteriol: 2011, 193(19);5335-46
[PubMed:21784929]
[WorldCat.org]
[DOI]
(I p)
Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969]
[WorldCat.org]
[DOI]
(I p)
B R Belitsky, J Brill, E Bremer, A L Sonenshein
Multiple genes for the last step of proline biosynthesis in Bacillus subtilis.
J Bacteriol: 2001, 183(14);4389-92
[PubMed:11418582]
[WorldCat.org]
[DOI]
(P p)
