GuaB

• Description: IMP dehydrogenase
  
  

Gene name	guaB
Synonyms	guaA
Essential	yes PubMed
Product	IMP dehydrogenase
Function	biosynthesis of GMP
Metabolic function and regulation of this protein in SubtiPathways: Purine synthesis, Nucleotides (regulation)
MW, pI	52 kDa, 6.168
Gene length, protein length	1464 bp, 488 aa
Immediate neighbours	yaaC, dacA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes, phosphoproteins

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU00090

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Inosine 5'-phosphate + NAD⁺ + H₂O = xanthosine 5'-phosphate + NADH (according to Swiss-Prot)

• Protein family: IMPDH/GMPR family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated (STY) PubMed, S-cysteinlyation after diamide stress (Cys-308) PubMed, PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1VRD (from Thermotoga maritima msb8, 60% identity, 80% similarity)

• UniProt: P21879

• KEGG entry: [3]

• E.C. number: 1.1.1.205

Expression and regulation

• Operon: guaB

• Sigma factor:

• Regulation:
  • activated during growth in the presence of branched chain amino acids (CodY) PubMed
  • inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´

• Regulatory mechanism:
  • CodY: transcription activation PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:
  • purification from B. subtilis with an N-terminal Strep-tag, for SPINE, (in pGP380): pGP901, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

PubMed