AbrB

• Description: transcriptional regulator of transition state genes
  
  

Gene name	abrB
Synonyms	cpsX, tolB
Essential	no
Product	transcriptional regulator
Function	regulation of gene expression during the transition from growth to stationary phase
Metabolic function and regulation of this protein in SubtiPathways: Biofilm, His, Asp, Asn, Ammonium/ glutamate, Central C-metabolism, Sugar catabolism
MW, pI	10 kDa, 6.57
Gene length, protein length	282 bp, 94 aa
Immediate neighbours	yabC, metS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00370

Phenotypes of a mutant

No swarming motility on B medium. PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): Abh, SpoVT (only N-terminal domain)

Genes/ operons controlled by AbrB

• Activated by AbrB: citB, hpr, rbsR-rbsK-rbsD-rbsA-rbsC-rbsB

• Repressed by AbrB: abrB, aprE, comK, ftsA-ftsZ, kinB, pbpE, sigH, spoVG, spo0E, tycA, sbo-alb, yqxM-sipW-tasA, sdpA operon, sunA, skfA operon, sinI-sinR, sigW, yuaB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on Ser-86 PubMed

• Cofactor(s):

• Effectors of protein activity: interaction with AbbA results in inactivation of AbrB PubMed

• Interactions: AbrB-AbbA PubMed; AbrB-AbrB PubMed, AbrB-Abh PubMed

• Localization:

Database entries

• Structure: 1Z0R (N-terminal DNA recognition domain), NCBI PubMed

• UniProt: P08874

• KEGG entry: [3]

Expression and regulation

• Operon: abrB PubMed

• Sigma factor: SigA PubMed

• Regulation: expressed at the onset of stationary phase PubMed
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism: repressed by Spo0A-P PubMed
  • Spo0A: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: TT731 (aphA3)

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

Mark Strauch, Baltimore, USA homepage

References

Reviews

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Daniel Schultz, Peter G Wolynes, Eshel Ben Jacob, José N Onuchic
Deciding fate in adverse times: sporulation and competence in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2009, 106(50);21027-34
[PubMed:19995980] [WorldCat.org] [DOI] (I p)

Z E V Phillips, M A Strauch
Bacillus subtilis sporulation and stationary phase gene expression.
Cell Mol Life Sci: 2002, 59(3);392-402
[PubMed:11964117] [WorldCat.org] [DOI] (P p)

Wolfgang Klein, Mohamed A Marahiel
Structure-function relationship and regulation of two Bacillus subtilis DNA-binding proteins, HBsu and AbrB.
J Mol Microbiol Biotechnol: 2002, 4(3);323-9
[PubMed:11931565] [WorldCat.org] (P p)

Original Publications

Other publications: PubMed