MalP

• Description: maltose-specific phosphotransferase system, EIICB of the PTS
  
  

Gene name	malP
Synonyms	glvC
Essential	no
Product	maltose-specific phosphotransferase system, EIICB
Function	maltose uptake and phosphorylation
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	57 kDa, 8.872
Gene length, protein length	1581 bp, 527 aa
Immediate neighbours	glvR, yfiB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU08200

Phenotypes of a mutant

no growth on maltose as single carbon source PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, membrane proteins, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

• Protein family: PTS permease, glucose permease (Glc) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P54715

• KEGG entry: [2]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: malA-glvR-malP PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induction by maltose (GlvR) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • GlvR: transcription activation PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP110 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: