Difference between revisions of "PucR"
| Line 1: | Line 1: | ||
| − | * '''Description:''' transcriptional regulator of puc genes <br/><br/> | + | * '''Description:''' transcriptional regulator of ''puc'' genes <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator of puc genes | + | |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator of ''puc'' genes |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of purine utilization | |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of purine utilization | ||
| Line 90: | Line 90: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[pucR]]-[[pucJ]]-[[pucK]]-[[pucL]]-[[pucM]]'' {{PubMed|12029039}} |
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12029039}} |
| − | * '''Regulation:''' negative autoregulation | + | * '''Regulation:''' |
| + | ** negative autoregulation {{PubMed|12029039}} | ||
| − | * '''Regulatory mechanism:''' repression by '''PucR''' | + | * '''Regulatory mechanism:''' |
| + | ** repression by '''PucR''' {{PubMed|12029039}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 15:07, 28 December 2009
- Description: transcriptional regulator of puc genes
| Gene name | pucR |
| Synonyms | yunI |
| Essential | no |
| Product | transcriptional regulator of puc genes |
| Function | regulation of purine utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation) | |
| MW, pI | 60 kDa, 6.188 |
| Gene length, protein length | 1593 bp, 531 aa |
| Immediate neighbours | pucH, pucJ |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU32420
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: cdaR family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O32138
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- negative autoregulation PubMed
- Regulatory mechanism:
- repression by PucR PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Jaclyn L Brandenburg, Lewis V Wray, Lars Beier, Hanne Jarmer, Hans H Saxild, Susan H Fisher
Roles of PucR, GlnR, and TnrA in regulating expression of the Bacillus subtilis ure P3 promoter.
J Bacteriol: 2002, 184(21);6060-4
[PubMed:12374841]
[WorldCat.org]
[DOI]
(P p)
Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039]
[WorldCat.org]
[DOI]
(P p)
A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136]
[WorldCat.org]
[DOI]
(P p)
