Difference between revisions of "CwlC"
| Line 90: | Line 90: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''cwlC'' {{PubMed|8407798}} |
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigK]] {{PubMed|8407798}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** expressed late during sporulation in the mother cell ([[SigK]]) {{PubMed|8407798}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| Line 120: | Line 121: | ||
=References= | =References= | ||
| − | <pubmed>10945275,,16042392,11375403, </pubmed> | + | <pubmed>10945275,,16042392,11375403, 8407798 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:57, 18 October 2009
- Description: N-acetylmuramoyl-L-alanine amidase
| Gene name | cwlC |
| Synonyms | |
| Essential | no |
| Product | N-acetylmuramoyl-L-alanine amidase |
| Function | mother cell lysis |
| MW, pI | 27 kDa, 5.939 |
| Gene length, protein length | 765 bp, 255 aa |
| Immediate neighbours | ymaB, spoVK |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU17410
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)
- Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: secreted (according to Swiss-Prot)
Database entries
- Structure: 1X60 (peptidoglycan binding domain)
- UniProt: Q06320
- KEGG entry: [3]
- E.C. number: 3.5.1.28
Additional information
Expression and regulation
- Operon: cwlC PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Masaki Mishima, Toshio Shida, Kazuto Yabuki, Ken-Ichi Kato, Junichi Sekiguchi, Chojiro Kojima
Solution structure of the peptidoglycan binding domain of Bacillus subtilis cell wall lytic enzyme CwlC: characterization of the sporulation-related repeats by NMR.
Biochemistry: 2005, 44(30);10153-63
[PubMed:16042392]
[WorldCat.org]
[DOI]
(P p)
T Shida, H Hattori, F Ise, J Sekiguchi
Mutational analysis of catalytic sites of the cell wall lytic N-acetylmuramoyl-L-alanine amidases CwlC and CwlV.
J Biol Chem: 2001, 276(30);28140-6
[PubMed:11375403]
[WorldCat.org]
[DOI]
(P p)
T Shida, H Hattori, F Ise, J Sekiguchi
Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
Biosci Biotechnol Biochem: 2000, 64(7);1522-5
[PubMed:10945275]
[WorldCat.org]
[DOI]
(P p)
A Kuroda, Y Asami, J Sekiguchi
Molecular cloning of a sporulation-specific cell wall hydrolase gene of Bacillus subtilis.
J Bacteriol: 1993, 175(19);6260-8
[PubMed:8407798]
[WorldCat.org]
[DOI]
(P p)
