Difference between revisions of "BsrF"

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(Biological materials)
(Biological materials)
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* '''Mutants:''' DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery available in the  [[Sabine Brantl]] lab
 
* '''Mutants:''' DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery available in the  [[Sabine Brantl]] lab
  
* '''Expression vectors:''' pWSR2, multi-copy vector for tetracycline-inducible expression of bsrF (kanamycin resistance gene)
+
* '''Expression vectors:'''  
  pUCB2SR2, multi-copy vector for constitutive expression of bsrF under its own promoter (kanamycin resistance gene) available in the [[Sabine Brantl]] lab
+
** pWSR2, multi-copy vector for tetracycline-inducible expression of ''bsrF'' (kanamycin resistance gene), available in the [[Sabine Brantl]] lab
 +
** pUCB2SR2, multi-copy vector for constitutive expression of ''bsrF'' under its own promoter (kanamycin resistance gene), available in the [[Sabine Brantl]] lab
 
          
 
          
* '''lacZ fusions:''' pACG1 to pACG19 containing regions of different size upstream of the bsrF promoter available in the [[Sabine Brantl]] lab
+
* '''lacZ fusions:''' pACG1 to pACG19 containing regions of different size upstream of the ''bsrF'' promoter available in the [[Sabine Brantl]] lab
  
 
=Labs working on this gene/RNA=
 
=Labs working on this gene/RNA=

Revision as of 17:06, 14 July 2009

  • Description: small RNA

Gene name bsrF
Synonyms SR2
Essential no
Product small RNA
Function unknown
Gene length bp
Immediate neighbours yobO, csaA
Gene sequence (+200bp)
Genetic context
File:GenE context.gif








The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: no entry
  • Structure:

Additional information

Expression and regulation

  • Regulatory mechanism: CodY: transcription activation PubMed
  • Additional information:

Biological materials

  • Mutants: DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery available in the Sabine Brantl lab
  • Expression vectors:
    • pWSR2, multi-copy vector for tetracycline-inducible expression of bsrF (kanamycin resistance gene), available in the Sabine Brantl lab
    • pUCB2SR2, multi-copy vector for constitutive expression of bsrF under its own promoter (kanamycin resistance gene), available in the Sabine Brantl lab
  • lacZ fusions: pACG1 to pACG19 containing regions of different size upstream of the bsrF promoter available in the Sabine Brantl lab

Labs working on this gene/RNA

  • Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
  • Kouji Nakamura, Tsukuba, University, Japan

Your additional remarks

References