NrdI

• Description: ribonucleoside-diphosphate reductase
  
  

Gene name	nrdI
Synonyms	ymaA
Essential	yes PubMed
Product	ribonucleoside-diphosphate reductase
Function	synthesis of deoxyribonucleoside triphosphates
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation)
MW, pI	14 kDa, 8.58
Gene length, protein length	390 bp, 130 aa
Immediate neighbours	ymzA, nrdE
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU17370

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nrdI family (according to Swiss-Prot)

• Paralogous protein(s): YosM

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1RLJ

• Swiss prot entry: P50618

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nrdI-nrdE-nrdF-ymaB PubMed

• Sigma factor:

• Regulation: distinct promoters for aerobic and anaerobic expression PubMed, anaerobic expression requires ResD PubMed
  • expressed under anaerobic conditions (ResD) PubMed

• Regulatory mechanism: ResD: transcription activation PubMed
  • ResD: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Elisabeth Härtig, Anja Hartmann, Manuela Schätzle, Alessandra M Albertini, Dieter Jahn
The Bacillus subtilis nrdEF genes, encoding a class Ib ribonucleotide reductase, are essential for aerobic and anaerobic growth.
Appl Environ Microbiol: 2006, 72(8);5260-5
[PubMed:16885274] [WorldCat.org] [DOI] (P p)

C Scotti, A Valbuzzi, M Perego, A Galizzi, A M Albertini
The Bacillus subtilis genes for ribonucleotide reductase are similar to the genes for the second class I NrdE/NrdF enzymes of Enterobacteriaceae.
Microbiology (Reading): 1996, 142 ( Pt 11);2995-3004
[PubMed:8969495] [WorldCat.org] [DOI] (P p)