XynB

• Description: xylan beta-1,4-xylosidase
  
  

Gene name	xynB
Synonyms	ynaK
Essential	no
Product	xylan beta-1,4-xylosidase
Function	xylan degradation
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	61 kDa, 5.326
Gene length, protein length	1599 bp, 533 aa
Immediate neighbours	xynP, xylR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU17580

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 43 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1YIF

• UniProt: P94489

• KEGG entry: [3]

• E.C. number: 3.2.1.37

Expression and regulation

• Operon: xynP-xynB

• Sigma factor: SigA

• Regulation: repressed by glucose (CcpA) , carbon catabolite repression (CcpA), induction by xylose (XylR)

• Regulatory mechanism: CcpA: transcription repression, CcpA: transcription repression, XylR: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

C Lindner, J Stülke, M Hecker
Regulation of xylanolytic enzymes in Bacillus subtilis.
Microbiology (Reading): 1994, 140 ( Pt 4);753-7
[PubMed:8012596] [WorldCat.org] [DOI] (P p)