Difference between revisions of "BsrG"

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* '''Description:''' small RNA <br/><br/>
+
* '''Description:''' hydrophobic toxin; interferes with cell envelope biosynthesis, causes membrane invaginations together with delocalization of the [[cell wall synthesis]] machinery, triggers autolysis, and inhibits protein biosynthesis <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || small RNA
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|style="background:#ABCDEF;" align="center"| '''Product''' || class I toxin
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
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|style="background:#ABCDEF;" align="center"|'''Function''' ||unknown
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Gene length''' ||  bp
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' ||   
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yolA]]'', ''[[yokL]]''
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 294 bp, 38 aa
 
|-
 
|-
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE.txt    Gene sequence      (+200bp)  ]'''  
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yolA]]'', ''[[SR4]]''
 
|-
 
|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:genE_context.gif]]
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein <br/>'''
 +
|-
 +
|colspan="2" | '''Genetic context''' <br/> [[Image:bsrG_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
  
−
<br/><br/>
+
<br/><br/><br/><br/><br/>
 +
= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[toxins, antitoxins and immunity against toxins]]}},
 +
{{SubtiWiki category|[[SPß prophage]]}},
 +
{{SubtiWiki category|[[membrane proteins]]}}
 +
 
 +
= This gene is a member of the following [[regulons]] =
 +
 
  
−
<br/><br/><br/><br/><br/>
 
 
=The gene=
 
=The gene=
  
 
=== Basic information ===
 
=== Basic information ===
  
−
* '''Coordinates:'''
+
* '''Locus tag:'''
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=?&redirect=T ?]
  
 
* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
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* '''SubtiList entry:''' no entry
 
* '''SubtiList entry:''' no entry
 
   
 
   
−
* '''Structure:'''  
+
=== Additional information===
 +
 
 +
 
 +
=The protein=
 +
 
 +
=== Basic information/ Evolution ===
 +
 
 +
* '''Catalyzed reaction/ biological activity:'''
 +
 
 +
* '''Protein family:'''
 +
 
 +
* '''Paralogous protein(s):'''
 +
 
 +
=== Extended information on the protein ===
 +
 
 +
* '''Kinetic information:'''
 +
 
 +
* '''Domains:'''
 +
 
 +
* '''Modification:'''
 +
 
 +
* '''Cofactor(s):'''
 +
 
 +
* '''Effectors of protein activity:'''
 +
 
 +
* '''[[SubtInteract|Interactions]]:'''
 +
 
 +
* '''[[Localization]]:'''
 +
** membrane protein (based on the presence of a trans-membrane domain)  {{PubMed|22229825}}
 +
 
 +
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=?&redirect=T ?]
 +
 
 +
* '''Structure:'''
 +
 
 +
* '''UniProt:'''
 +
 
 +
* '''KEGG entry:'''
 +
 
 +
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
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=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:'''  
+
* '''Operon:''' ''[[bsrG]]''  {{PubMed|22229825}}
  
−
* '''Sigma factor:'''  
+
* '''Sigma factor:''' [[SigA]]  {{PubMed|22229825}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** expression increases at the onset of the stationary phase {{PubMed|22229825}}
 +
** the mRNA is destabilized upon interaction with the antitoxin RNA [[SR4]] {{PubMed|22229825}}
 +
** the mRNA is degraded by [[Rnc]], [[Rny]] and [[Rnr]] {{PubMed|22229825}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** the mRNA is degraded by [[Rnc]], [[Rny]] and [[Rnr]], degradation is enhanced at higher temperature {{PubMed|22229825}}
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  
 +
** half-life of the mRNA: 15 min  {{PubMed|22229825}}
  
 
=Biological materials =
 
=Biological materials =
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* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
  
 +
=Labs working on this gene/protein=
  
−
=Labs working on this gene/RNA=
+
[[Sabine Brantl]], Jena, Germany
−
 
 
−
Kouji Nakamura, Tsukuba, University, Japan
 
  
 
=Your additional remarks=
 
=Your additional remarks=
  
 
=References=
 
=References=
 +
<pubmed>18948176, 20525796 23969414 22229825 26234942</pubmed>
  
−
# Saito S, Kakeshita H, Nakamura K (2009) Novel small RNA-encoding genes in the intergenic regions of ''Bacillus subtilis''. ''Gene'' '''428:''' 2-8. [http://www.ncbi.nlm.nih.gov/sites/entrez/18948176 PubMed]
+
[[Category:Protein-coding genes]]

Latest revision as of 08:21, 4 August 2015

  • Description: hydrophobic toxin; interferes with cell envelope biosynthesis, causes membrane invaginations together with delocalization of the cell wall synthesis machinery, triggers autolysis, and inhibits protein biosynthesis

Gene name bsrG
Synonyms
Essential no
Product class I toxin
Function unknown
MW, pI
Gene length, protein length 294 bp, 38 aa
Immediate neighbours yolA, SR4
Get the DNA and protein
Genetic context
File:BsrG context.gif
This image was kindly provided by SubtiList






Categories containing this gene/protein

toxins, antitoxins and immunity against toxins, SPß prophage, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

Database entries

  • BsubCyc: ?
  • DBTBS entry: no entry
  • SubtiList entry: no entry

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • BsubCyc: ?
  • Structure:
  • UniProt:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expression increases at the onset of the stationary phase PubMed
    • the mRNA is destabilized upon interaction with the antitoxin RNA SR4 PubMed
    • the mRNA is degraded by Rnc, Rny and Rnr PubMed
  • Regulatory mechanism:
    • the mRNA is degraded by Rnc, Rny and Rnr, degradation is enhanced at higher temperature PubMed
  • Additional information:
    • half-life of the mRNA: 15 min PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:

Labs working on this gene/protein

Sabine Brantl, Jena, Germany

Your additional remarks

References

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