Difference between revisions of "MhqA"

From SubtiWiki
Jump to: navigation, search
(New page: * '''Description:''' write here <br/><br/> {| align="right" border="1" cellpadding="2" |- |style="background:#ABCDEF;" align="center"|'''Gene name''' |''ykcA'' |- |style="background:#ABC...)
 
 
(41 intermediate revisions by 6 users not shown)
Line 1: Line 1:
−
* '''Description:''' write here <br/><br/>
+
* '''Description:''' hydroquinone-specific dioxygenase, confers resistence to methyl-hydroxyquinone <br/><br/>
−
 
 
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''ykcA''
+
|''mhqA ''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykcA''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Essential''' ||  
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' ||  
+
|style="background:#ABCDEF;" align="center"| '''Product''' || hydroquinone-specific dioxygenase
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || resistence to methyl-hydroxyquinone
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU12870 mhqA]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.404   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 5.404   
Line 18: Line 19:
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 948 bp, 316 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 948 bp, 316 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||  
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[steT]]'', ''[[ykcB]]''
 +
|-
 +
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU12870 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU12870 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU12870 DNA_with_flanks]
 +
|-
 
|-
 
|-
−
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ykcA_nucleotide.txt    Gene sequence      (+200bp)  ]'''
 
−
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ykcA_protein.txt Protein sequence]'''
 
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ykcA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ykcA_context.gif]]
 +
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 +
|-
 +
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mhqA_1353080_1354030_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:mhqA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU12870]]
 
|-
 
|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
  
 
<br/><br/>
 
<br/><br/>
 +
 +
= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[resistance against oxidative and electrophile stress]]}}
 +
 +
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[MhqR regulon]]}}
  
 
=The gene=
 
=The gene=
Line 35: Line 52:
 
=== Basic information ===
 
=== Basic information ===
  
−
* '''Coordinates:'''
+
* '''Locus tag:''' BSU12870
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU12870&redirect=T BSU12870]
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ykcA.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mhqA.html]
  
 
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13228]
 
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13228]
  
 
=== Additional information===
 
=== Additional information===
 +
 +
  
  
Line 54: Line 74:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
−
* '''Protein family:'''
+
* '''Protein family:''' extradiol ring-cleavage dioxygenase family (according to UniProt)
  
−
* '''Paralogous protein(s):'''
+
* '''Paralogous protein(s):''' [[MhqE]], [[MhqO]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 70: Line 90:
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
−
* '''Interactions:'''
+
* '''[[SubtInteract|Interactions]]:'''
  
−
* '''Localization:'''
+
* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU12870&redirect=T BSU12870]
  
−
* '''Structure:'''
+
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3OAJ 3OAJ] ([[MhqO]], 34% identical residues)
  
−
* '''Swiss prot entry:'''
+
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34689 O34689]
  
−
* '''KEGG entry:'''
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU12870]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 88: Line 109:
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:'''  
+
* '''Operon:''' ''mhqA'' {{PubMed|17725564}}
  
−
* '''Sigma factor:'''  
+
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mhqA_1353080_1354030_1 mhqA] {{PubMed|22383849}}
 +
 
 +
* '''Sigma factor:''' [[SigA]] {{PubMed|17725564}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** induced by catechol and 2-methylhydroquinone ([[MhqR]]) {{PubMed|17725564}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[MhqR]]: transcription repression {{PubMed|17725564}}
  
−
* '''Additional information:'''  
+
* '''Additional information:'''
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 183 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =
Line 118: Line 144:
 
=References=
 
=References=
  
−
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
+
<pubmed>17407181,17725564,, </pubmed>
 +
 
 +
[[Category:Protein-coding genes]]

Latest revision as of 08:58, 25 June 2019

  • Description: hydroquinone-specific dioxygenase, confers resistence to methyl-hydroxyquinone

Gene name mhqA
Synonyms ykcA
Essential no
Product hydroquinone-specific dioxygenase
Function resistence to methyl-hydroxyquinone
Gene expression levels in SubtiExpress: mhqA
MW, pI 35 kDa, 5.404
Gene length, protein length 948 bp, 316 aa
Immediate neighbours steT, ykcB
Sequences Protein DNA DNA_with_flanks
Genetic context
YkcA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MhqA expression.png
























Categories containing this gene/protein

resistance against oxidative and electrophile stress

This gene is a member of the following regulons

MhqR regulon

The gene

Basic information

  • Locus tag: BSU12870

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: extradiol ring-cleavage dioxygenase family (according to UniProt)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3OAJ (MhqO, 34% identical residues)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by catechol and 2-methylhydroquinone (MhqR) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 183 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Retrieved from "http://subtiwiki.uni-goettingen.de/wiki//index.php?title=MhqA&oldid=210472"