Difference between revisions of "ComN"

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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of competence and DNA uptake
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of competence and DNA uptake
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27660 comN]
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=ComN ComN]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 4.622   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 4.622   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[secDF]]'', ''[[spoVB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[secDF]]'', ''[[spoVB]]''
 
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14726&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27660 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27660 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27660 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yrzD_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yrzD_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|-
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comN_2829152_2829448_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:comN_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27660]]
 
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|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
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<br/><br/><br/><br/>
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<br/><br/><br/><br/>
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<br/><br/><br/><br/>
  
 
<br/><br/>
 
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27660&redirect=T BSU27660]
  
 
* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
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=== Additional information===
 
=== Additional information===
−
 
−
 
−
 
  
 
=The protein=
 
=The protein=
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
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* '''Catalyzed reaction/ biological activity:''' post-transcriptional control of ''[[comEA]]'' expression  [http://www.ncbi.nlm.nih.gov/sites/entrez/19028902 PubMed]
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* '''Catalyzed reaction/ biological activity:'''  
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** post-transcriptional control of ''[[comEA]]'' expression  [http://www.ncbi.nlm.nih.gov/sites/entrez/19028902 PubMed]
 +
** promotes the accumulation of its target ''[[comEA]]'' mRNA to septal and polar sites {{PubMed|22582279}}
 
* '''Protein family:'''
 
* '''Protein family:'''
  
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
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* '''Interactions:'''
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* '''[[SubtInteract|Interactions]]:'''
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** [[DivIVA]]-[[ComN]] {{PubMed|22582279}}
  
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* '''Localization:''' cell membrane (according to Swiss-Prot)
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* '''[[Localization]]:'''
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** localizes to the division site and cell poles in a [[DivIVA]] dependent fashion {{PubMed|22582279}}
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27660&redirect=T BSU27660]
  
 
* '''Structure:'''
 
* '''Structure:'''
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* '''Operon:'''  
 
* '''Operon:'''  
  
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* '''[[Sigma factor]]:'''  
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=comN_2829152_2829448_-1 comN] {{PubMed|22383849}}
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 +
* '''Sigma factor:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
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* '''Additional information:'''  
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* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 140 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
  
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<pubmed>19028902,,19028902, </pubmed>
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<pubmed>19028902,22582279, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Latest revision as of 09:57, 17 April 2014

  • Description: mediates post-transcriptional control of comE operon expression

Gene name comN
Synonyms yrzD
Essential no
Product post-transcriptional regulator of comE expression
Function regulation of competence and DNA uptake
Gene expression levels in SubtiExpress: comN
Interactions involving this protein in SubtInteract: ComN
MW, pI 11 kDa, 4.622
Gene length, protein length 294 bp, 98 aa
Immediate neighbours secDF, spoVB
Sequences Protein DNA DNA_with_flanks
Genetic context
YrzD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ComN expression.png















Categories containing this gene/protein

genetic competence, membrane proteins

This gene is a member of the following regulons

The ComN regulon: comEA-comEB-comEC

The gene

Basic information

  • Locus tag: BSU27660

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • post-transcriptional control of comEA expression PubMed
    • promotes the accumulation of its target comEA mRNA to septal and polar sites PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 140 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Valquiria Tiago dos Santos, Alexandre W Bisson-Filho, Frederico J Gueiros-Filho
DivIVA-mediated polar localization of ComN, a posttranscriptional regulator of Bacillus subtilis.
J Bacteriol: 2012, 194(14);3661-9
[PubMed:22582279] [WorldCat.org] [DOI] (I p)

Mitsuo Ogura, Teruo Tanaka
The Bacillus subtilis late competence operon comE is transcriptionally regulated by yutB and under post-transcription initiation control by comN (yrzD).
J Bacteriol: 2009, 191(3);949-58
[PubMed:19028902] [WorldCat.org] [DOI] (I p)

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