Difference between revisions of "BsrF"
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<br/><br/><br/><br/><br/>  | <br/><br/><br/><br/><br/>  | ||
| + | = [[Categories]] containing this gene/protein =  | ||
| + | {{SubtiWiki category|[[ncRNA]]}}  | ||
| + | |||
| + | = This gene is a member of the following [[regulons]] =  | ||
| + | |||
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=The gene=  | =The gene=  | ||
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=== Database entries ===  | === Database entries ===  | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=?&redirect=T ?]  | ||
* '''DBTBS entry:''' no entry  | * '''DBTBS entry:''' no entry  | ||
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=== Additional information===  | === Additional information===  | ||
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=Biological materials =  | =Biological materials =  | ||
| − | * '''  | + | * '''Mutants:''' DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery available in the  [[Sabine Brantl]] lab  | 
| − | * '''Expression vector  | + | * '''Expression vectors:'''   | 
| + | ** pWSR2, multi-copy vector for tetracycline-inducible expression of ''bsrF'' (kanamycin resistance gene), available in the [[Sabine Brantl]] lab  | ||
| + | ** pUCB2SR2, multi-copy vector for constitutive expression of ''bsrF'' under its own promoter (kanamycin resistance gene), available in the [[Sabine Brantl]] lab  | ||
| − | * '''lacZ   | + | * '''lacZ fusions:''' pACG1 to pACG19 containing regions of different size upstream of the ''bsrF'' promoter available in the [[Sabine Brantl]] lab  | 
| − | |||
=Labs working on this gene/RNA=  | =Labs working on this gene/RNA=  | ||
| − | [[Sabine Brantl |Sabine Brantl]], Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany [http://www.uni-jena.de/AG_Bakteriengenetik_(_Doz__Dr__Sabine_Brantl_).html homepage]  | + | * [[Sabine Brantl |Sabine Brantl]], Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany [http://www.uni-jena.de/AG_Bakteriengenetik_(_Doz__Dr__Sabine_Brantl_).html homepage]  | 
| − | Kouji Nakamura, Tsukuba, University, Japan  | + | * Kouji Nakamura, Tsukuba, University, Japan  | 
=Your additional remarks=  | =Your additional remarks=  | ||
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<pubmed>18948176, 19542274 </pubmed>  | <pubmed>18948176, 19542274 </pubmed>  | ||
| + | |||
| + | [[Category:small RNA]]  | ||
Latest revision as of 15:23, 2 April 2014
-  Description: small RNA 
 
| Gene name | bsrF | 
| Synonyms | SR2 | 
| Essential | no | 
| Product | small RNA | 
| Function | unknown | 
| Gene length | bp | 
| Immediate neighbours | yobO, csaA | 
| Gene sequence (+200bp) | |
|  Genetic context  File:GenE context.gif  | |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag:
 
Phenotypes of a mutant
Database entries
- BsubCyc: ?
 
- DBTBS entry: no entry
 
- SubtiList entry: no entry
 
- Structure:
 
Additional information
Expression and regulation
- Operon: bsrF PubMed
 
- Additional information:
 
Biological materials
- Mutants: DB104::Delta bsrF::cat, DB104::Delta bsrF::ery, 168::Delta bsrF::cat, 168::Delta bsrF::ery available in the Sabine Brantl lab
 
-  Expression vectors: 
- pWSR2, multi-copy vector for tetracycline-inducible expression of bsrF (kanamycin resistance gene), available in the Sabine Brantl lab
 - pUCB2SR2, multi-copy vector for constitutive expression of bsrF under its own promoter (kanamycin resistance gene), available in the Sabine Brantl lab
 
 
- lacZ fusions: pACG1 to pACG19 containing regions of different size upstream of the bsrF promoter available in the Sabine Brantl lab
 
Labs working on this gene/RNA
- Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
 - Kouji Nakamura, Tsukuba, University, Japan
 
Your additional remarks
References
Heike Preis, Rita A Eckart, Rajani K Gudipati, Nadja Heidrich, Sabine Brantl  
CodY activates transcription of a small RNA in Bacillus subtilis. 
J Bacteriol: 2009, 191(17);5446-57 
[PubMed:19542274]
  [WorldCat.org]
 [DOI]
 (I p)
Shinichi Saito, Hiroshi Kakeshita, Kouji Nakamura  
Novel small RNA-encoding genes in the intergenic regions of Bacillus subtilis. 
Gene: 2009, 428(1-2);2-8 
[PubMed:18948176]
  [WorldCat.org]
 [DOI]
 (P p)