OdhB

• Description: 2-oxoglutarate dehydrogenase complex (dihydrolipoamide transsuccinylase, E2 subunit)
  
  

Gene name	odhB
Synonyms	citM
Essential	yes PubMed
Product	2-oxoglutarate dehydrogenase complex (dihydrolipoamide transsuccinylase, E2 subunit)
Function	TCA cycle
MW, pI	45 kDa, 4.859
Gene length, protein length	1251 bp, 417 aa
Immediate neighbours	odhA, yocS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU19360

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Succinyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-succinyldihydrolipoyl)lysine (according to Swiss-Prot)

• Protein family: sodium:bile acid symporter family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: OdhA-OdhB-PdhD

• Localization: cell membrane (according to Swiss-Prot), membrane associated PubMed

Database entries

• Structure:

• Swiss prot entry: P16263

• KEGG entry: [3]

• E.C. number: 2.3.1.61 1

Expression and regulation

• Operon: odhA-odhB

• Sigma factor: SigA

• Regulation: repressed by glucose (2.4-fold) (CcpA) PubMed, constitutive

• Regulatory mechanism: CcpA: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • pGP1145 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Stülke lab)

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

References

1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
2. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
3. Kobayashi et al. (2003) Essential Bacillus subtilis genes. Proc. Natl. Acad. Sci. USA 100: 4678-4683. PubMed
4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed