Difference between revisions of "GlnA"
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| − | * '''Description:''' glutamine synthetase <br/><br/> | + | * '''Description:''' trigger enzyme: glutamine synthetase and effector of [[TnrA]] and [[GlnR]] <br/><br/> |
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| − | <pubmed>11719184, 12139611, 11741602, 2573733, 8636055, 19233925, 16493705, 16885465, 6141156</pubmed> | + | <pubmed>18195355, 11719184, 12139611, 11741602, 2573733, 8636055, 19233925, 16493705, 16885465, 6141156</pubmed> |
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | ||
Revision as of 10:08, 30 May 2009
| Gene name | glnA |
| Synonyms | |
| Essential | no |
| Product | trigger enzyme: glutamine synthetase |
| Function | glutamine biosynthesis, control of TnrA and GlnR activity |
| MW, pI | 50 kDa, 4.874 |
| Gene length, protein length | 1332 bp, 444 aa |
| Immediate neighbours | glnR, ynxB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
auxotrophic for glutamine
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine (according to Swiss-Prot)
- Protein family: glutamine synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: K(M) for: Glu: 27 mM, ATP: 2.4 mM, ammonium: 0.18 mM; v(max): 3.7 µmol/min/mg
- Domains: glutamate binding flap (aa 300 ... 306: protects unstable intermediates from abberant hydrolysis)
- Modification: phosphorylated on ser/ thr/ tyr PubMed
- Cofactor(s): Mg(2+)
- Effectors of protein activity: feedback inhibition by glutamine, glutamine binds thhe entrance site for glutamate
- Interactions: TnrA-GlnA, GlnR-GlnA, (only the feedback-inhibited enzyme interacts with TnrA and GlnR)
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- Swiss prot entry: P12425
- KEGG entry: BSU17460
- E.C. number: 6.3.1.2
Additional information
GlnA is a homooligomer of 12 subunits
Expression and regulation
- Regulation: expressed in the absence of glutamine PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody: available in Karl Forchhammer lab
Labs working on this gene/protein
Susan Fisher, Boston, USA homepage
Your additional remarks
References
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
