Difference between revisions of "Sandbox"

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* '''Description:''' sulfate adenylyltransferase <br/><br/>
+
* '''Description:''' adenylyl-sulfate kinase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''sat''
+
|''cysC''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylnB ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylnC ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || sulfate adenylyltransferase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || adenylyl-sulfate kinase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || sulfate activation
+
|style="background:#ABCDEF;" align="center"|'''Function''' || sulfate reduction and activation
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 5.646  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 5.083  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1146 bp, 382 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 591 bp, 197 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cysP]]'', ''[[cysC]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sat]]'', ''[[ylnD]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13433&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13434&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:sat_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:cysC_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 43: Line 43:
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cysHP-sat-cysC-ylnDEF.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cysHP-sat-cysC-ylnDEF.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13378]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13379]
  
 
=== Additional information===
 
=== Additional information===
Line 54: Line 54:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:''' sulfate adenylyltransferase family (according to Swiss-Prot)
+
* '''Protein family:''' APS kinase family (according to Swiss-Prot)
  
* '''Paralogous protein(s):''' [[YitA]]
+
* '''Paralogous protein(s):'''
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 78: Line 78:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34764 O34764]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34577 O34577]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15590]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15600]
  
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.7.4 2.7.7.4]
+
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.25 2.7.1.25]
  
 
=== Additional information===
 
=== Additional information===

Revision as of 23:00, 11 May 2009

  • Description: adenylyl-sulfate kinase

Gene name cysC
Synonyms ylnC
Essential no
Product adenylyl-sulfate kinase
Function sulfate reduction and activation
MW, pI 22 kDa, 5.083
Gene length, protein length 591 bp, 197 aa
Immediate neighbours sat, ylnD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CysC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: APS kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: repressed by casamino acids PubMed, repressed in the presence of cysteine (CymR)
  • Regulatory mechanism: CymR: transcription repression, S-box Element: transcriptional antitermination
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

Your additional remarks

References

  1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed