Difference between revisions of "Sandbox"

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* '''Description:''' involved in isopentenol (isoprenoid) biosynthesis <br/><br/>
+
#redirect[[ccpC]]
 
 
{| align="right" border="1" cellpadding="2"
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|''yhfR''
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || isoprenoid biosynthesis
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 5.164 
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 579 bp, 193 aa
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhfQ]]'', ''[[yhfS]]''
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12874&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yhfR_context.gif]]
 
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|}
 
 
 
__TOC__
 
 
 
<br/><br/>
 
 
 
=The gene=
 
 
 
=== Basic information ===
 
 
 
* '''Coordinates:'''
 
 
 
===Phenotypes of a mutant ===
 
 
 
no detectable phenotype  [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]
 
 
 
=== Database entries ===
 
 
 
* '''DBTBS entry:''' no entry
 
 
 
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13062]
 
 
 
=== Additional information===
 
 
 
 
 
=The protein=
 
 
 
=== Basic information/ Evolution ===
 
 
 
* '''Catalyzed reaction/ biological activity:''' unknown  [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]
 
 
 
* '''Protein family:''' GpmB subfamily (according to Swiss-Prot) similar to 2,3-diphosphoglycerate-dependent phosphoglycerate mutases  [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]
 
 
 
* '''Paralogous protein(s):'''
 
 
 
=== Extended information on the protein ===
 
 
 
* '''Kinetic information:'''
 
 
 
* '''Domains:'''
 
 
 
* '''Modification:'''
 
 
 
* '''Cofactor(s):'''
 
 
 
* '''Effectors of protein activity:'''
 
 
 
* '''Interactions:'''
 
 
 
* '''Localization:'''
 
 
 
=== Database entries ===
 
 
 
* '''Structure:'''
 
 
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O07617 O07617]
 
 
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU10340]
 
 
 
* '''E.C. number:'''
 
 
 
=== Additional information===
 
 
 
=Expression and regulation=
 
 
 
* '''Operon:'''
 
 
 
* '''[[Sigma factor]]:'''
 
 
 
* '''Regulation:'''
 
 
 
* '''Regulatory mechanism:'''
 
 
 
* '''Additional information:''' weakly expressed  [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]
 
 
 
=Biological materials =
 
 
 
* '''Mutant:'''
 
 
 
* '''Expression vector:'''
 
       
 
* '''lacZ fusion:'''
 
 
 
* '''GFP fusion:'''
 
 
 
* '''two-hybrid system:'''
 
 
 
* '''Antibody:'''
 
 
 
=Labs working on this gene/protein=
 
 
 
=Your additional remarks=
 
 
 
=References=
 
 
 
# Pearson CL, Loshon CA, Pedersen LB, Setlow B, Setlow P (2000) Analysis of the function of a putative 2,3-diphosphoglyceric acid-dependent phosphoglycerate mutase from ''Bacillus subtilis''. ''J. Bacteriol.'' '''182:''' 4121-4123. [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 

Revision as of 14:07, 8 May 2009

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