Difference between revisions of "Sandbox"

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* '''Description:''' subunit of DNA topoisomerase IV <br/><br/>
+
* '''Description:''' endo-1,4-beta-glucanase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''parC''
+
|''bglC''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''grlA ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''eglS ''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || subunit of DNA topoisomerase IV
+
|style="background:#ABCDEF;" align="center"| '''Product''' || endo-1,4-beta-glucanase)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || chromosome segregation and compaction
+
|style="background:#ABCDEF;" align="center"|'''Function''' || beta-1,4-glucan degradation
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 91 kDa, 6.307  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 55 kDa, 8.619  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2418 bp, 806 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1497 bp, 499 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[parE]]'', ''[[ynfC]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[alsT]]'', ''[[ynfE]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13693&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12135&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
 
|-
 
|-
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:parC_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:bglC_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
  
<br/><br/><br/><br/>
+
<br/><br/>
  
 
=The gene=
 
=The gene=
Line 40: Line 40:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
  
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/parEC.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bglC.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11803]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10437]
  
 
=== Additional information===
 
=== Additional information===
Line 60: Line 58:
 
* '''Protein family:'''
 
* '''Protein family:'''
  
* '''Paralogous protein(s):''' [[GyrA]]
+
* '''Paralogous protein(s):'''
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 76: Line 74:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:''' cytoplasm (according to Swiss-Prot),  Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] polar localization in actively growing cells [http://www.ncbi.nlm.nih.gov/sites/entrez/9539793 PubMed]
+
* '''Localization:''' extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1WP5 1WP5] (C-Terminal domain, Geobacillus stearothermophilus)
+
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1H2J 1H2J] (from B. Agaradhaerence)
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q45066 Q45066]
+
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU18100]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU18130]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 122: Line 120:
 
=References=
 
=References=
  
# Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in ''Bacillus subtilis'': identification of new proteins at the DNA replication factory ''Proteomics'' '''6:''' 2135-2146. [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
+
# Voigt et al. (2009) Cell physiology and protein secretion of ''Bacillus licheniformis'' compared to ''Bacillus subtilis''. ''J Mol Microbiol Biotechnol.'' '''16:''' 53-68 [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 15:26, 5 May 2009

  • Description: endo-1,4-beta-glucanase

Gene name bglC
Synonyms eglS
Essential no
Product endo-1,4-beta-glucanase)
Function beta-1,4-glucan degradation
MW, pI 55 kDa, 8.619
Gene length, protein length 1497 bp, 499 aa
Immediate neighbours alsT, ynfE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
BglC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: extracellular (signal peptide) PubMed

Database entries

  • Structure: 1H2J (from B. Agaradhaerence)
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed