Difference between revisions of "Sandbox"

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−
* '''Description:''' RNA polymerase sporulation mother cell-specific (early) [[sigma factor]] SigE <br/><br/>
+
* '''Description:''' RNA polymerase sporulation forespore-specific (late) [[sigma factor]] SigG <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''sigE''
+
|''sigG''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spoIIGB ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spoIIIG ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase sporulation mother cell-specific (early) [[sigma factor]] SigE
+
|style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase sporulation forespore-specific (late) [[sigma factor]] SigG
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || transcription of sporulation genes (early mother cell)
+
|style="background:#ABCDEF;" align="center"|'''Function''' || transcription of sporulation genes (late forespore)
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 9.212  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.496  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 717 bp, 239 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 780 bp, 260 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIIGA]]'', ''[[sigG]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sigE]]'', ''[[ylmA]]''
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13406&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13407&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
−
|-
+
|colspan="2" | '''Genetic context''' <br/> [[Image:sigG_context.gif]]
−
|-
 
−
|colspan="2" | '''Genetic context''' <br/> [[Image:sigE_context.gif]]
 
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoIIGA-sigE-sigG.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoIIGA-sigE-sigG.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10235]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10236]
  
 
=== Additional information===
 
=== Additional information===
Line 54: Line 52:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
−
* '''Catalyzed reaction/ biological activity:''' RNA polymerase [[sigma factor]], active in the mother cell
+
* '''Catalyzed reaction/ biological activity:'''  
  
 
* '''Protein family:'''
 
* '''Protein family:'''
Line 60: Line 58:
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
  
−
=== Genes controlled by SigE ===
+
=== Genes controlled by SigG ===
  
−
''[[dacB]], [[pbpI]], [[spoVD]]'', ''[[phoB]]-[[ydhF]]'', ''[[asnO]]'', ''[[bdbD]]-[[bdbC]]'', ''[[bofA]]'', ''[[cotE]]'', ''[[ctaE]]'', ''[[yaaH]]'', ''[[ybaK]]-[[cwlD]]''
+
''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'', ''[[sigG]]'', ''[[pbpF]], [[pbpG]]'', ''[[spoVAA]]-[[spoVAB]]-[[spoVAC]]-[[spoVAD]]-[[spoVAE]]-[[spoVAF]]-[[lysA]]'', ''[[sleB]]-[[ypeB]]'', ''[[sspF]]'', ''[[ybaK]]-[[cwlD]]'', ''[[sspL]]''
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
−
* '''Interactions:''' [[Processing]]
+
* '''Interactions:''' [[SpoIIAB]]-[[SigG]]
  
 
* '''Localization:'''
 
* '''Localization:'''
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* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15320]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15330]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 95: Line 93:
  
 
* '''Operon:''' ''[[spoIIGA]]-[[sigE]]-[[sigG]]''
 
* '''Operon:''' ''[[spoIIGA]]-[[sigE]]-[[sigG]]''
 +
''[[sigG]]''
  
−
* '''[[Sigma factor]]:''' [[SigA]]
+
* '''[[Sigma factor]]:''' ''[[spoIIGA]]'': [[SigA]], ''[[sigG]]'': [[SigF]], [[SigG]]
  
−
* '''Regulation:''' expressed early during sporulation ([[Spo0A]])
+
* '''Regulation:''' spoIIGA: expressed early during sporulation ([[Spo0A]])
 +
''[[sigG]]'': expressed during sporulation
  
 
* '''Regulatory mechanism:''' activated by [[Spo0A]], repression by [[SinR]]
 
* '''Regulatory mechanism:''' activated by [[Spo0A]], repression by [[SinR]]

Revision as of 12:07, 28 April 2009

  • Description: RNA polymerase sporulation forespore-specific (late) sigma factor SigG

Gene name sigG
Synonyms spoIIIG
Essential no
Product RNA polymerase sporulation forespore-specific (late) sigma factor SigG
Function transcription of sporulation genes (late forespore)
MW, pI 29 kDa, 5.496
Gene length, protein length 780 bp, 260 aa
Immediate neighbours sigE, ylmA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SigG context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Genes controlled by SigG

dacF-spoIIAA-spoIIAB-sigF, sigG, pbpF, pbpG, spoVAA-spoVAB-spoVAC-spoVAD-spoVAE-spoVAF-lysA, sleB-ypeB, sspF, ybaK-cwlD, sspL

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

sigG

  • Regulation: spoIIGA: expressed early during sporulation (Spo0A)

sigG: expressed during sporulation

  • Regulatory mechanism: activated by Spo0A, repression by SinR
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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